Mass spectrometry method for detecting klebsiella pneumoniae in ready-to-eat fresh fruits and vegetables

Abstract

The invention relates to a method for detecting klebsiella pneumoniae in a ready-to-eat fruit and vegetable agricultural product by mass spectrometry. The method comprises the following steps: S1, preparing klebsiella pneumoniae in ready-to-eat fruits and vegetables by specific selective culture; S2, establishing a method for separating klebsiella pneumoniae from fruits and vegetables; S3, extracting and separating suspected klebsiella pneumoniae protein components from fruits and vegetables; and S4, collecting a klebsiella pneumoniae protein spectrum by using matrix desorption ionization time-of-flight mass spectrometry, and performing identifying by adopting a characteristic peak fitting method. The core technology includes research and development of klebsiella pneumoniae selective medium components on the surfaces of ready-to-eat fruits and vegetables and establishment of a separation method, establishment of a pretreatment method of microbial mass spectrometry identification and establishment of microbial mass spectrometry conditions. According to the method for detecting and identifying the klebsiella pneumoniae in the ready-to-eat fruits and vegetables without standard agricultural products, the klebsiella pneumoniae in the ready-to-eat fruits and vegetables is detected and identified by adopting biochemical selective culture in combination with a mass spectrometry method, so that the klebsiella pneumoniae in the fruits and vegetables of agricultural products is accurately identified, more fruit and vegetable microorganism protein data information is obtained, and data is provided for traceability and pathopoiesis analysis of the klebsiella pneumoniae in fruits and vegetables.

Description

technical field [0001] The invention relates to the field of microbial detection of agricultural products and identification of microorganisms by mass spectrometry, more particularly, the detection of food-borne pathogenic microorganisms of ready-to-eat fresh fruits and vegetables of agricultural products. Background technique [0002] At present, there are mainly four types of detection methods for food-borne microorganisms. The first is the traditional conventional detection methods. The detection methods mainly include bacteria enrichment, separation, biochemical identification, etc. The detection steps are relatively complicated and the cycle is long, and it takes 5-10 days. However, the traditional method is mature in technology and requires simple equipment. The traditional method is currently the mainstream method for microbial detection, but there is no mature and standardized detection method for Klebsiella pneumoniae in food in my country. The second is molecular bi...

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a mass spectrometry method for detecting Klebsiella pneumoniae in ready-to-eat fresh fruits and vegetables, to solve the problem of Klebsiella pneumoniae detection in instant fruits and vegetables

Images