Seed production method of oyster high-temperature-resistant strain

A technology of high temperature resistance and oysters, which is applied in the fields of fish farming, application, and climate change adaptation. It can solve the problems of small oyster germplasm size, difficult breeding of offspring, and poor nutritional foundation, so as to shorten the breeding cycle and improve high temperature resistance. Ability, the effect of rapid high temperature resistance

Active Publication Date: 2020-11-10
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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AI Technical Summary

Problems solved by technology

Conventional family breeding can improve the high temperature tolerance of oysters to a certain extent, but there are problems such as long cycle, high cost and low efficiency
However, the oyster germplasm in the i

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0019] (1) In March 2017, 980 oysters with a shell height of 1.5-2 cm and no damage to the shell were selected as the parents of the core basic population in the high tide area of ​​the intertidal zone in Huangdao District, Qingdao. Parents are placed in a breeding cage with a pore size of 1 cm, and the subtidal sea area with rich single-cell bait and low wind and waves is selected in Jiaonan to fatten the parents, so as to improve the nutritional level of the parents in the intertidal zone, thereby increasing the number and quality of their offspring. survival rate. In May, the parents were transferred to the holding pond of the shellfish nursery factory in Laizhou for 7 days. The holding conditions were water temperature 18-20°C, salinity 30, 50% water change every day, and bait feeding once every 3 hours. . When the gonads are mature, the running water in the shade is used to stimulate spawning, and the core basic population is constructed by the method of group self-propa...

Embodiment 2

[0025] 1) In March 2017, 1,000 oysters with undamaged shells were selected as the parents of the core basic population in the intertidal zone of Huangdao District, Qingdao. The parent is placed in a breeding cage with a pore size of 1 cm, and the fattening of the parent is carried out in the subtidal sea area where the single-cell bait is abundant and the wind and waves are small in Jiaonan. In May, the parents were transferred to the holding pond of the shellfish nursery factory in Laizhou for 7 days. The holding conditions were water temperature 18-20°C, salinity 30, 50% water change every day, and bait feeding once every 3 hours. . When the gonads are mature, the running water in the shade is used to stimulate spawning, and the core basic population is constructed by the method of group self-propagation. When eye spots appear, the ctenophore shells are used as the attachment base for juveniles to collect seedlings.

[0026] 2) When the core base population grows to a shell...

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PUM

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Abstract

The invention belongs to the field of shellfish genetic breeding in the marine biotechnology, and particularly relates to a seed production method of an oyster high-temperature-resistant strain. Intertidal zone oysters with the shell height larger than 1.5 cm and without damage serve as parents of a core basic group, the parents are fattened in a subtidal zone sea area in a group self-propagationmanner and then bred to obtain a basic group, and then each generation of young oysters are repeatedly placed in an intertidal zone in summer to be subjected to temperature shock stress; and group breeding is carried out after fattening breeding is carried out in the subtidal zone sea area, and then the high-temperature-resistant strain with genetic stability is obtained. According to the method,the technical difficulties of poor nutritional basis, low egg laying amount, breeding difficulty and the like of the oysters in the intertidal zone are overcome, the phenotypic plasticity of the resistance traits of the oysters in the extreme environment of the intertidal zone is utilized in the seed production process, the breeding efficiency is improved, the breeding period is shortened, and thesurvival rate of the oysters in the high-temperature period in summer is remarkably increased. The invention provides a new way for cultivating a new high-temperature-resistant oyster variety, and can also provide a new improved variety for the oyster cultivation industry.

Description

technical field [0001] The invention belongs to the field of shellfish genetics and breeding in marine biotechnology, and in particular relates to a seed production method of oyster high temperature resistant strains. Background technique [0002] Oysters are naturally distributed in the mid-tidal zone to the subtidal zone in the intertidal zone, and are highly susceptible to extreme environmental impacts. Oysters are prone to large-scale mortalities in summer, causing heavy losses to the industry. High temperature is one of the most important environmental factors leading to this problem, and cultivating high temperature resistant strains is an effective technical approach. Conventional family breeding can improve the high temperature tolerance of oysters to a certain extent, but there are problems such as long cycle, high cost and low efficiency. However, because oyster germplasm in the intertidal zone has problems such as small size, poor nutritional foundation, and dif...

Claims

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Application Information

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IPC IPC(8): A01K61/54
CPCA01K61/54Y02A40/81
Inventor 丛日浩王鲁平李莉张国范王威
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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