A kind of preparation method of adipose tissue frozen section

A technology of adipose tissue and frozen section, which is applied in the field of adipose tissue section, which can solve the problems of affecting pathological diagnosis, deformation of tissue cells, and chipping, and achieve the effects of lowering temperature, enriching fibers, and increasing hardness

Active Publication Date: 2022-04-05
THE FIRST AFFILIATED HOSPITAL OF XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The structural characteristics of adipose tissue determine that it is difficult to cut a complete and flat slice of adipose tissue during the frozen section process. At present, the most commonly used method is to push or pull the slice after thick sectioning, resulting in serious deformation and overlapping of tissue cells. Even the slides will seriously affect the pathological diagnosis. Frozen sectioning of adipose tissue is one of the most difficult problems in the field of pathology. There has been no good experimental method for a long time, which has caused great trouble to pathological technology and diagnosis. In serious cases, it will cause misdiagnosis or missed diagnosis

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0019] The present invention discloses a method for preparing a frozen section of adipose tissue, which adopts an existing frozen section machine and a quick-freezing table, and specifically includes the following steps:

[0020] (1) Pre-cool the cryostat and the quick-freezing table, set the temperature of the cryostat box to -35°C and the temperature of the quick-freeze table to -40°C.

[0021] (2) Place the adipose tissue in the center of the frozen holder, and drip the embedding medium on the holder around the adipose tissue, where the size of the adipose tissue is smaller than the size of the frozen holder. Among them, the size of adipose tissue was 1×1×0.5 cm, and the embedding medium was ordinary glue or OCT embedding medium.

[0022] (3) Put the holder on the quick-freezing table for freezing treatment. When the top part of the adipose tissue is close to completely frozen (usually it takes 4-6 minutes to freeze), use the freezing hammer on the quick-freezing table to p...

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Abstract

The invention relates to a method for preparing a frozen section of adipose tissue, which comprises a frozen section machine and a quick-freezing platform for pre-cooling, placing the adipose tissue in the center of a freezing support, placing the support on a quick-freezing platform for freezing treatment, and then Fixed in the chuck of the cryostat machine for trimming, adjusting the direction of tissue sectioning, quick freezing again, sectioning and other treatments; finally, patching, fixing, staining and sealing were performed to complete the preparation of frozen sections of adipose tissue. The invention can prepare complete and smooth frozen sections of adipose tissue.

Description

technical field [0001] The invention relates to a method for slicing adipose tissue, in particular to a method for preparing a frozen slice of adipose tissue. Background technique [0002] Frozen section is a method in which the tissue reaches a certain hardness with the help of low temperature conditions, and then slices, which can well preserve the antigen and enzyme activity of the tissue, so it is widely used in clinical rapid pathological diagnosis and scientific research of diseases. Different tissues will be sectioned differently. Adipose tissue is composed of a large number of clusters of adipocytes separated into lobules by thin layers of loose connective tissue. Reticular fibers in adipose tissue are well developed, but there is little intercellular substance in adipose tissue. The structural characteristics of adipose tissue determine that it is difficult to cut a complete and flat section of adipose tissue in the process of frozen sectioning. At present, the mo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/06G01N1/28G01N1/42
CPCG01N1/06G01N1/28G01N1/42
Inventor 黄红浪付莉李卫华张维肖凌云汤雅玲陈双龙宋冰洁童安祺
Owner THE FIRST AFFILIATED HOSPITAL OF XIAMEN UNIV
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