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Application of lycopene in preparation of antiviral drugs

A technology of tomatine and virus, which is applied in the direction of antiviral agents, pharmaceutical formulations, medical preparations containing active ingredients, etc.

Active Publication Date: 2020-11-13
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problem of the urgent need for broad-spectrum specific antiviral drugs in the above prior art, the application provides an application of tomatine in inhibiting cysteine ​​protease activity

Method used

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  • Application of lycopene in preparation of antiviral drugs
  • Application of lycopene in preparation of antiviral drugs
  • Application of lycopene in preparation of antiviral drugs

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0025] 1 Materials and methods

[0026] 1.1 Cells and viruses

[0027] Vero cells, ST cells, Macr-145 cells and BHK-21 cells are preserved by our laboratory. PEDV MS, YZ, SH and CV777 strains passed on Vero cells, TGEV JS2012 passed on ST cells, PRRSV BB0907 passed on Marc-145 cells, EMCV NJ08 and SVA CH-SD passed on BHK-21 cells were all obtained from this Laboratory preservation.

[0028] 1.2 Main reagents

[0029] Tomatidine used in in vitro experiments was purchased from Selleck Chemicals with a purity of >99%.

[0030] 1.3 Cell Viability Detection

[0031] Vero cells were cultured with DMEM-10%FBS containing different concentrations of tomatine, and incubated at 37°C for 48h. Cell viability was detected with an enhanced CCK8 kit (Beyotime). GraphPad Prism 6.0 software was used to calculate the tomatine concentration (CC 50 ). At the same time, DMSO was used as a negative control.

[0032] 1.4 PEDV infection inhibition test

[0033] The effect of tomatine on the ...

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Abstract

The invention relates to the technical field of biology, in particular to application of lycopene in preparation of antiviral drugs. The lycopene has a new function of inhibiting cysteine protease activity, has a broad-spectrum inhibition effect on viruses containing 3CLpro, and can be used for research and development of antiviral drugs.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an application of tomatine in the preparation of antiviral drugs. Background technique [0002] Porcine epidemic diarrhea virus (PEDV) is a single-stranded positive-sense RNA virus belonging to the Coronaviridae family, which mainly causes watery diarrhea in infected pigs. In 2012, the prevailing strain of PEDV in my country mutated, causing an outbreak in pig populations across the country, and commercial vaccines could not provide effective protection, causing serious economic losses. In 2013, the mutant strain of PEDV was introduced into the United States and spread rapidly to 16 states in the United States, causing severe diarrhea in piglets in large-scale pig farms and huge economic losses. To date there is no effective vaccine. The PEDV genome encodes a cysteine ​​protease (Nsp5), called 3CL protease (3CLpro), which plays an important role in viral replication. There are man...

Claims

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Application Information

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IPC IPC(8): A61K31/58A61P31/14
CPCA61K31/58A61P31/14Y02A50/30
Inventor 姜平王鹏程王先炜白娟范慧
Owner NANJING AGRICULTURAL UNIVERSITY
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