Bjerkandera adusta G14 and application thereof

A technology of tobacco tube bacteria and plant pathogenic bacteria, applied in the field of environmental microorganisms, can solve problems such as single function of strains, and achieve the effects of promoting growth, strong phosphorus-dissolving ability, and high development and utilization value

Active Publication Date: 2020-11-13
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, most of the isolated strains are monofunctional, and there are few strains with multiple functions at the same time.

Method used

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  • Bjerkandera adusta G14 and application thereof
  • Bjerkandera adusta G14 and application thereof
  • Bjerkandera adusta G14 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: pipe bacteria ( Bjerkandera adusta , G14) isolation, identification and preservation

[0029] 1.1 Medium and its preparation

[0030] PD medium: peel 200g of fresh potatoes, boil 1.1L of distilled water for 30min, filter through 4 layers of gauze to obtain potato liquid, add 0.05g of chloramphenicol to the potato liquid, and distilled water to dissolve to 1L;

[0031] PDB medium: add 20g / L glucose to PD medium;

[0032] PDA medium: add 20g / L agar powder to PDB medium;

[0033] Carbon source screening medium: on the basis of PD medium, 20g / L carbon source (glucose, maltose, sucrose, soluble starch, fructose) is added, and the pH is naturally added;

[0034] Nitrogen source screening medium: based on PDB medium, 1g / L nitrogen source (ammonium nitrate, ammonium chloride, ammonium sulfate, urea, peptone), natural pH;

[0035] Metal ion screening medium: on the basis of PDB medium, add 0.5mmol / L metal source (CuS , MnS , CaC , MgS , ZnS and FeC ...

Embodiment 2

[0045] Embodiment 2: pipe bacteria ( Bjerkandera adusta , G14) Determination of antibacterial properties

[0046] The plate confrontation method was used to determine the antibacterial activity of the strain against six kinds of plant pathogenic bacteria tested. First, all endophytic fungal strains and pathogenic bacteria are activated, and then in the ultra-clean workbench, punch holes on the fresh mycelium of the endophytic fungal strains and pathogenic bacteria edges activated in the PDA plate with a hole puncher with a diameter of 6mm, and respectively punch The bacterial cake obtained from the hole was inoculated on a PDA plate with a diameter of 90 mm. The inoculation point was on both sides of the medium, on the same diameter 20 mm from the edge of the plate, and three replicates were set up as the experimental group, and the pathogenic bacteria were only inoculated on the right side of the plate as the control group. , and set up 3 repetitions, and then place the exp...

Embodiment 3

[0047] Embodiment 3: pipe bacteria ( Bjerkandera adusta , G14) Determination of pro-proliferative properties

[0048] Endophyte Phosphorus Solubilization Screening:

[0049] The isolated and purified blueberry endophytic fungi were activated and cultured, and the activated endophytic fungi were picked (d=0.6cm) and inoculated on PKO inorganic phosphorus medium and Montina organic phosphorus medium respectively, and the inoculation After placing the last disposable culture dish in a constant temperature incubator at 28 °C for 72 hours, observe whether the transparent phosphorus-dissolving circle appears or not to determine whether the strain has phosphorus-dissolving effect, and measure and calculate the diameter of the phosphorus-dissolving circle D of the strain. Value and the ratio of the colony diameter d value, preliminarily judge whether the strain has phosphorus-solubilizing ability. The experiment shows that the D / d value of G14 in Montina medium is up to 1.7, which ...

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Abstract

The invention discloses a strain of Bjerkandera adusta G14 and application thereof. The Bjerkandera adusta G14 was preserved in China General Microbiological Culture Collection Center on July 6, 2020,and the preservation number is CGMCC No.20218. The Bjerkandera adusta G14 disclosed by the invention is diversified in application functions, has good biocontrol, growth promotion and decoloration functions, and has relatively high development and utilization value for organisms and the environment.

Description

technical field [0001] The invention belongs to the technical field of environmental microbes, and relates to a pipe fungus G14 and its application. Background technique [0002] Plant endophytic fungi (Plant endophytic fungi) commonly exist in healthy plant tissues, and refer to those that spend a certain stage or all stages of their life history in various tissues and organs of healthy plants, and the host does not show any symptoms. Fungioid. It is considered to be one of the important sources of biological control factors for plant diseases. It can directly face the infection of some pathogenic bacteria, attack the pathogen itself or the pathogenic factor to degrade it, produce antagonistic substances, or induce the plant itself to produce systemic resistance to suppress pathogens, etc., while the genes of the plant itself still maintain natural traits , is a natural resource bank for biological control of plant diseases. Endophytic fungi exist in most plants and are ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C02F3/34A01N63/30A01P21/00C02F101/30C12R1/645
CPCC12N1/14C02F3/342A01N63/30C02F2101/308C12R2001/645C12N1/145
Inventor 侯瑞尚晓静张富美
Owner GUIZHOU UNIV
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