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A method and system for simulating macrophages to improve acute kidney injury experiments in mice

A technology of acute kidney injury and macrophages, applied in the field of experimental teaching, can solve the problems of high cost, time-consuming reproduction, inconvenient teaching of popular science, etc., and achieve the effect of improving experimental skills, improving sense of substitution, and reducing experimental errors

Active Publication Date: 2022-06-03
WEST CHINA HOSPITAL SICHUAN UNIV
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Problems solved by technology

[0004] The purpose of the present invention is to provide a method and system for simulating macrophages to improve acute kidney injury experiments in mice, aiming to solve the existing macrophage intervention effect and molecular mechanism on acute kidney injury in mice is complex and requires operators It has high experimental ability, and at the same time, it takes a long time to reproduce the experiment, and it costs a lot of money, which is not convenient for the technical problems of science popularization and teaching

Method used

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  • A method and system for simulating macrophages to improve acute kidney injury experiments in mice
  • A method and system for simulating macrophages to improve acute kidney injury experiments in mice
  • A method and system for simulating macrophages to improve acute kidney injury experiments in mice

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Embodiment Construction

[0051] S2.5, formulate and identify the steps of the induced differentiation product.

[0055] CD86 and CD206 are markers for M1 and M2 macrophages, respectively. The results of flow phenotyping showed that: M0 and M2

[0065] After both kidneys were continuously clamped for 30 min, the arterial clamp was removed.

[0067] S3.3, simulate the experimental process of constructing ischemia-reperfusion-induced acute kidney injury model.

[0080] S5, obtain the pathological tissue detection data of the mouse sample, and provide an experimental summary.

[0081] Specifically, the pathological tissue detection data of the mouse sample is displayed by means of pictures, audio and text.

[0087] M2 promotes metatubular regenerative repair in ischemia-reperfusion-induced acute kidney injury.

[0094] M2 promotes hypoxia-reoxygenation-induced proliferation of mouse primary renal tubular epithelial cells.

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Abstract

The invention provides a method for simulating macrophages to improve acute kidney injury experiments in mice, belonging to the field of experimental teaching, including selecting the type of mice; preparing simulated macrophages; and constructing a type of mouse kidney injury model, wherein, The mouse kidney injury model includes an acute kidney injury model induced by ischemia-reperfusion and an oxidative injury model of mouse primary renal tubular epithelial cells induced by hypoxia and reoxygenation; Intervention; obtain the pathological tissue detection data of mouse samples, and give the experimental summary. The invention provides a method for simulating macrophages to improve acute kidney injury experiments in mice, which can assist in memorizing standard experimental steps, improve the experimental skills of users, and is also more conducive to the popularization and teaching of the experiment. The invention also provides a system for simulating macrophages to improve acute kidney injury experiments in mice.

Description

A method and system for simulating macrophages to improve acute kidney injury experiments in mice technical field The invention belongs to the field of experimental teaching, more specifically, relate to a kind of simulated macrophage to improve mouse acute Methods and systems for kidney injury experiments. Background technique Acute kidney injury is one of clinical common critical illness, mainly manifests as the rapid decline of renal function in the short term, has High morbidity and mortality, and a risk of developing chronic kidney disease and accelerated end-stage renal disease. Although currently A variety of treatments have been validated in animal models of acute kidney injury, but there is still no effective drug to treat acute kidney injury The progress of the disease, therefore, requires in-depth research on the pathogenesis and prevention mechanisms based on an understanding of its pathophysiology. Macrophages are one of the most important immune cell...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G09B23/36
CPCG09B23/36
Inventor 王成世
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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