A method and system for simulating macrophages to improve acute kidney injury experiments in mice

Abstract

The invention provides a method for simulating macrophages to improve acute kidney injury experiments in mice, belonging to the field of experimental teaching, including selecting the type of mice; preparing simulated macrophages; and constructing a type of mouse kidney injury model, wherein, The mouse kidney injury model includes an acute kidney injury model induced by ischemia-reperfusion and an oxidative injury model of mouse primary renal tubular epithelial cells induced by hypoxia and reoxygenation; Intervention; obtain the pathological tissue detection data of mouse samples, and give the experimental summary. The invention provides a method for simulating macrophages to improve acute kidney injury experiments in mice, which can assist in memorizing standard experimental steps, improve the experimental skills of users, and is also more conducive to the popularization and teaching of the experiment. The invention also provides a system for simulating macrophages to improve acute kidney injury experiments in mice.

Description

A method and system for simulating macrophages to improve acute kidney injury experiments in mice technical field The invention belongs to the field of experimental teaching, more specifically, relate to a kind of simulated macrophage to improve mouse acute Methods and systems for kidney injury experiments. Background technique Acute kidney injury is one of clinical common critical illness, mainly manifests as the rapid decline of renal function in the short term, has High morbidity and mortality, and a risk of developing chronic kidney disease and accelerated end-stage renal disease. Although currently A variety of treatments have been validated in animal models of acute kidney injury, but there is still no effective drug to treat acute kidney injury The progress of the disease, therefore, requires in-depth research on the pathogenesis and prevention mechanisms based on an understanding of its pathophysiology. Macrophages are one of the most important immune cell...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method and system for simulating macrophages to improve acute kidney injury experiments in mice, aiming to solve the existing macrophage intervention effect and molecular mechanism on acute kidney injury in mice is complex and requires operators It has high experimental ability, and at the same time, it takes a long time to reproduce the experiment, and it costs a lot of money, which is not convenient for the technical problems of science popularization and teaching

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