Array type in-vitro micro-magnetic stimulation device suitable for cell electrical activity regulation and control

Abstract

The invention discloses an array type in-vitro micro-magnetic stimulation device suitable for cell electrical activity regulation and control. The device is of great significance for researching regulation and control rules of hippocampus cell SchafferCA1 synaptic plasticity. Firstly, design of a submillimeter-size array type mMS device needs to be completed, the part focuses on researching inductance coil parameters and an array arrangement mode of the device, solves the key problems of biocompatibility, safety, interference resistance, a driving module and the like of the device, and meets the requirement that magnetic stimulation and microelectrode array electrophysiological records (MEA2100-60, German) are synchronously completed on a rat hippocampus in-vitro brain slice; and the regulation and control rule of single-point stimulation on the hippocampus SchafferCA1 synaptic plasticity is studied, a PP-DG-CA3-CA1 neural pathway is subjected to multi-point magnetic stimulation, and finally, the modulation rule of mMS on the hippocampus Schaffer-CA1 synaptic plasticity is disclosed.

Description

technical field [0001] The present invention designs a new submillimeter-sized array-type isolated micro-magnetic stimulation device, which can realize precise regulation of cell electrical activity, and has the characteristics of multi-point synergistic stimulation, flexible control, and strong targeting. The present invention belongs to fields of biomedical engineering. Background technique [0002] Electromagnetic stimulation technology is currently a major means of diagnosis, treatment and research of neuropsychiatric diseases. As a form of Electrical Stimulation (ES) technology, Deep Brain Stimulation (DBS) uses stereotaxic surgery to precisely implant stimulating electrodes into the targeted areas of the brain to generate stimulating signals to interfere with abnormal neural electrical activity. , has been proven to play a role in the treatment of neuropsychiatric diseases including epilepsy, major depression, and obsessive-compulsive disorder; and Transcranial Magnet...

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Problems solved by technology

[0003]Although there is some understanding of the effects of DBS and TMS on brain activity and how these effects change corresponding behaviors, there are still some technical and biological differences in their application. learning restrictions

Magnetic resonance imaging of patients treated with DBS may lead to excessive heating at the end of the stimulating electrode, resulting in nerve damage. The DBS stimulating electrode directly contacts biological tissue. Inflammation and immune response of the tissue may alter the therapeutic effect

The TMS coil is larger (size: 10-20cm), the magnetic field strength will decrease with the square of the field source distance, it is difficult to locate the deep target area of ​​the brain, it has a lower spatial resolution, and the TMS device needs to be strong enough Magnetic fields (e.g., >1T) penetrate the skull to activate deep neural tissue and are therefore difficult to control in experimental settings

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