Automatic solution replacing device for culture of three-dimensional organoid spheres

Abstract

The invention discloses an automatic solution replacing device for culture of three-dimensional organoid spheres. The automatic solution replacing device comprises a sample introduction container assembly and / or a sample introduction needle cleaning assembly, a sample introduction needle assembly, a sample application needle assembly and a spent solution collecting bottle, wherein one end of the sample introduction needle assembly is in pipeline connection with a sample application needle assembly, and the other end of the sample introduction needle assembly can selectively extend into the sample introduction container assembly or sample introduction needle cleaning assembly; and the sample application needle assembly comprises an aspirating needle and a sample application needle, the aspirating needle is connected with the spent solution collecting bottle through an aspirating peristaltic pump pipe, and the sample application needle is connected with the sample introduction needle assembly through a sample introduction peristaltic pump pipe. Through driving the aspirating needle and the sample application needle to perform solution replacing operation on organoid cell spheres separately by employing two sets of high-precision self-adapting peristaltic pumps, the flow rate of solution replacing can be accurately controlled, and injury to the cell spheres caused by uncontrollable fluid shearing stress is avoided as much as possible.

Description

technical field [0001] The invention relates to the technical field of biological cell culture, in particular to an automatic liquid exchange device for three-dimensional organoid spheroid culture in the aspect of organoid culture. Background technique [0002] Organoid is an in vitro 3D tissue culture technology that has been developed through breakthroughs in recent years. It can quickly cultivate human tissue cells in vitro with a high success rate, forming a "microtissue" that retains the tissue structure and biological information of the original organ. It has broad application prospects in the fields of chemotherapy and regenerative medicine. Tumor organoid culture technology is to establish a microenvironment similar to the body in vitro, and achieve 3D culture in vitro of tumor cells isolated from patient tumor tissues to form a miniature tumor model. Use this model to help tumor patients to test the sensitivity of multiple drugs in an individualized way, and realiz...

AI Technical Summary

Problems solved by technology

However, there is no automatic liquid exchange device specially designed for the cultivation of three-dimensional organoid cell spheroids on the market. Experimenters often use manual operations to avoid sucking or blowing the cultured organoid cell spheroids out of the designated area when changing the liquid. In the culture area, and when the pipette tip touches the cell ball or the cells on the outer surface of the cell ball are damaged or even necrotic due to high fluid shear force, extra care must be taken when manually replacing the culture medium or adding the drug solution, resulting in The efficiency of three-dimensional organoid cell spheroid culture is extremely low, which greatly limits the large-scale cultivation and application of organoid cell spheroids by researchers

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