Method for installing semen freezing fine tube into finger-shaped tube
A finger-shaped tube and fine tube technology, applied in the field of frozen semen, can solve the problems of hand frostbite, low tube installation efficiency, long time consumption, etc., and achieve the effects of reducing loss, improving tube installation efficiency, and preventing hand frostbite
Inactive Publication Date: 2020-11-27
ANHUI AGRICULTURAL UNIVERSITY
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Problems solved by technology
The traditional method needs to be operated in liquid nitrogen. It is a method of installing tubes in a horizontal direction. The operator needs to hang his hands directly above the liquid nitrogen to operate. And the consumption of liquid nitrogen is very large, which increases the production cost of frozen semen
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[0021] 1. Prepare thawing solution
[0022] ①Use an electronic balance to accurately weigh 2g of Tianyuan Ao Rui Freezing Essence Reagent E, dissolve it in 40mL of pure water, and stir it with a magnetic stirrer to fully dissolve it;
[0023] ② Use a 1000μL pipette to divide the prepared thawing solution into 15mL centrifuge tubes, 0.25mL frozen fine tube corresponds to 2.5mL thawing solution, and 0.5mL frozen fine tube corresponds to 5mL thawing solution;
[0024] ③Tighten the cap of the centrifuge tube with the packed thawing solution and place it in a 37°C water bath to preheat.
[0025] 2. Assemble the tube loading tool
[0026] ① Prepare a foam box with a length of 20cm, width of 10cm and depth of 15cm, two plastic cups with a diameter of 9cm and a depth of 12cm, and put the plastic cups into the foam box with the opening facing up;
[0027] ② Fill the assembled tube loader with liquid nitrogen, and the amount of liquid nitrogen should at least cover the mouth of the pl...
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The invention relates to a method for installing a semen freezing fine tube into a finger-shaped tube, which comprises the following specific steps of: (1) assembling a tubing tool, namely preparing afoam box and a plastic cup, and installing the plastic cup into the foam box with the opening of the plastic cup facing upwards, pouring liquid nitrogen into an assembled tubing device, and ensuringthat the quantity of the liquid nitrogen at least covers the opening of the plastic cup; (2) putting semen freezing fine tubes into the liquid nitrogen, namely putting semen freezing fine tubes frozenby a programmed freezing instrument or a fumigation method into the plastic cup filled with the liquid nitrogen, and enabling the tampon ends of the semen freezing fine tubes to face upward; and (3)loading the semen freezing fine tubes into a finger-shaped tube, namely clamping the finger-shaped tubes by using long tweezers, and slowly immersing the finger-shaped tubes into the liquid nitrogen,so as to enable the finger-shaped tubes to be completely immersed into the plastic cup filled with the semen freezing fine tubes in the liquid nitrogen; and lifting the semen freezing fine tubes one by one by using long tweezers and putting the semen freezing fine tubes into the finger-shaped tube with the tampon ends facing upwards. The method disclosed by the invention is a vertical tubing method, the tubing efficiency can be improved under the condition of not influencing the quality of frozen semen, safety accidents such as hand frostbite and the like are prevented, and the loss of liquidnitrogen is reduced.
Description
technical field [0001] The invention relates to the field of frozen sperm, in particular to a method for loading frozen sperm tubes into finger tubes. Background technique [0002] The reproductive performance of breeding males determines the production level of the breeding group to a large extent. As the saying goes, a good sow makes a good litter; a good boar makes a good slope. In the process of natural reproduction, a large number of breeding males need to be raised every year to meet the needs of sow mating in breeding enterprises, which greatly increases the breeding costs of enterprises. Although semen collection, dilution, room temperature preservation and artificial insemination techniques can improve the utilization efficiency of breeding males, reduce the number of breeding males, and indirectly improve the breeding efficiency of enterprises. However, the storage time of fresh semen at room temperature is limited, and the quality changes greatly during the stora...
Claims
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IPC IPC(8): A01N1/02
CPCA01N1/0252
Inventor 曹祖兵杨贤树张翔栋任尚张运海
Owner ANHUI AGRICULTURAL UNIVERSITY