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A kind of endophytic fungus Aspergillus rubrum and its application

An endophytic fungus and red ribbon technology, applied in the field of microorganisms, can solve the problems of high requirements for equipment and process conditions and high cost of enzyme production, and achieve the effect of stable enzyme activity and high enzyme activity.

Active Publication Date: 2022-01-11
武夷学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the technology of using microbial degumming to prepare bamboo fiber is to use a single biological enzyme or a composite biological enzyme to treat bamboo. The production cost of enzyme is high, and the requirements for equipment and process conditions are relatively high.

Method used

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  • A kind of endophytic fungus Aspergillus rubrum and its application
  • A kind of endophytic fungus Aspergillus rubrum and its application
  • A kind of endophytic fungus Aspergillus rubrum and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Screening of strains

[0026] 1. Primary screening of strains

[0027] (1) The newly collected wild reed branches, which are produced in Wuyishan City, Fujian Province, cut the reed leaves, branches and roots into 3-4 cm long, then washed them with detergent for 3 times, and then rinsed them with running water for 30 minutes. After washing, put them in sterilized jars according to the classification of leaves, branches and roots, and irradiate them with ultraviolet light for 30 minutes;

[0028] (2) Immerse in 70% ethanol for 30s (leaves for 10s) and pour off, rinse with sterile distilled water for 2 to 3 times, then immerse in 2.5% sodium hypochlorite solution for 5min (leaves for 3min) and pour off, then rinse with sterile distilled water for 2 ~3 times;

[0029] (3) Use a sterilized scalpel to peel off the outer skin of the root and stem, take the middle part of the rhizome, cut into a size of 0.5cm×0.5cm, and insert them into the PAD medium respectively; the endop...

Embodiment 2

[0044] Application of Aspergillus rubrum z4 in Degradation of Bamboo Powder

[0045] After activation, Aspergillus rubrum z4 was inoculated into potato culture medium, shaken at 30°C and 150 rpm for 48 hours to obtain seed liquid. The seed liquid was inoculated into the bamboo powder degradation medium according to the inoculum amount of 10%, and cultured with shaking at 30°C and 150r / min for 4 days. The degradation of bamboo powder components before and after fermentation was measured, and the results are shown in Table 1.

[0046] Bamboo powder degradation medium (g / L): bamboo powder 20.0, yeast extract 10.0, NaCl 2, KH 2 PO 4 1. MgSO 4 0.3, the initial pH of fermentation is natural.

[0047] Table 1 The degradation rate of different components of bamboo powder

[0048] Fat wax pectin Hemicellulose lignin Cellulose Degradation rate(%) 1.41 68.45 33.57 9.33 2.28

[0049] It can be seen from Table 1 that after fermentation by Aspergill...

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Abstract

The invention discloses an endophytic fungus Aspergillus rubrum and its application, belonging to the technical field of microorganisms. The microorganism is named as Aspergillus nomius z4, and it has been preserved in the China Center for Type Culture Collection with the preservation number: CCTCC NO:M 2019946 and the preservation date: November 18, 2019. Aspergillus rubrum z4 of the present invention uses liquid fermentation to produce pectinase, and can effectively degrade components such as pectin, hemicellulose, lignin, cellulose and wax in bamboo waste, and the microorganism can be applied to degrade bamboo powder Obtained in bamboo fiber.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to an endophytic fungus Aspergillus rubrum and its application. Background technique [0002] Pectin is a type of polysaccharide mainly composed of D-galacturonic acid and D-galacturonic acid methyl ester connected by α-1,4 glycosidic bonds, widely present in the primary wall of plant cell walls and the middle layer of cells , which acts to glue the cell walls together. Pectinase is a general term for a group of enzymes that can decompose pectin. Pectinase is mainly produced by plants and microorganisms, and bacteria, actinomycetes, yeast and mold can all produce pectinase in microorganisms. [0003] Bamboo industry is one of the four sunrise industries of my country's forestry industry. my country's bamboo resources are extremely rich, and bamboo species, distribution range and bamboo forest area rank first in the world. Bamboo is a perennial plant with short ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N9/88C12N9/26C12N9/18D01C1/04C12R1/66
CPCC12N9/88C12N9/2408C12N9/18C12Y301/01011C12Y302/01015C12Y402/02002D01C1/04C12R2001/66C12N1/145
Inventor 李碧婵李小青肖国利黄星星刘炳南尚保亚陈嘉发张敏
Owner 武夷学院
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