The invention discloses a MALDI-TOF
mass spectrum microbiological identification method based on the distance between spectrums, and the method comprises the following steps: 1, spectrum data preprocessing: carrying out denoising and baseline correction
processing on original
mass spectrum data, extracting and merging corrected data peaks, and carrying out normalization
processing to form a data
list; step 2, carrying out average reference spectrum construction on the data based on spectrum spacing dimension reduction, and collecting a plurality of representative spectrums for each
microorganism;
pairing the same spectrum by using a
peak value matching
algorithm; calculating the average position and intensity of each group of matching peaks to form an average reference spectrum; constructing an average reference spectrum, then carrying out inter-spectrum distance calculation, obtaining peaks corresponding to the sample spectrum and the average reference spectrum, then calculating an inter-peak distance, and generating a distance vector as a low-dimensional representation of the
microorganism sample spectrum; and step 3,
microorganism classification: performing
binary classification and multi-classification on the microorganism sample to complete microorganism identification.