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A strain of Bacillus ornithine suitable for the treatment of cyanide-containing wastewater

A technology of Bacillus ornithine and Bacillus, applied in the field of water treatment, can solve the problems of lack of experience in the application of halophilic microbial engineering, few successful cases, and microbial poisoning.

Active Publication Date: 2020-08-18
CHONGQING UNIV OF ARTS & SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] 2) In addition to having a rather high salinity and alkalinity, cartap wastewater also contains a large amount of toxic cyanide, which is toxic to microorganisms
[0009] 3) Most of the studies on alkali-resistant, salt-resistant, and cyanide-degrading microorganisms are only applicable to laboratory research, and there are few successful cases applicable to actual engineering, and there is insufficient experience in engineering application of salt-loving and tolerant microorganisms in environmental protection

Method used

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  • A strain of Bacillus ornithine suitable for the treatment of cyanide-containing wastewater

Examples

Experimental program
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Effect test

Embodiment 1

[0043] Embodiment 1: Production fermentation of B1132

[0044]B1132 can be produced by fermentation using common corn steep liquor as raw material. When 6% corn steep liquor and 0.1% dipotassium hydrogen phosphate are configured in a 500L fermenter, the pH=7. Inoculate 10% bacteria, stir at 200 rpm, and culture at 28°C for 48 hours to obtain the maximum biomass of B1132 bacteria, with a concentration of 10E9CFU / mL.

Embodiment 2

[0045] Example 2: Treatment of cyanide-containing wastewater from a pesticide factory

[0046] Collect the cyanide-containing waste water (pH = 10, salinity 3%) of thurendan and enter the biological oxidation tank, put in the Bacillus ornithini B1132 strain, the initial inoculum amount is 10% (the concentration of the bacterial solution is 10E9CFU / mL) . Aeration was carried out at room temperature to amplify the bacteria. After 48 hours, the bacteria density in the wastewater in the pool reached 10E9CFU / mL. Afterwards, the cyanide-containing waste water of thuramidan was continuously passed into the biological oxidation tank containing Bacillus ornithini B1132 for continuous treatment, and the residence time was 72 hours. After the above-mentioned treatment, the cyanide-containing wastewater of the pesticide factory was mixed with other low-concentration wastewater such as flushing wastewater. After subsequent conventional treatment, it has achieved discharge standards, and t...

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Abstract

The invention relates to the field of water treatment, and discloses a strain of Bacillus ornithinius that is suitable for treating cyanide-containing wastewater containing cyanide. It was preserved in the General Microbiology Center of the China Microbiological Culture Collection Management Committee, its preservation number is CGMCC18052, and the microbial classification is named Ornithinibacillus sp.; the 16s ribosomal subunit gene sequence of the ornithine B1132 is as follows: Shown in SEQ ID NO.1. The Bacillus ornithinius B1132 of the present invention has both alkali resistance, salt resistance, cyanide resistance and the ability to degrade cyanide, fully meets the conditions for treating cyanide-containing wastewater containing thurendan, and is an ideal strain for treating cyanide-containing wastewater containing thurendan resource.

Description

technical field [0001] The invention relates to the field of water treatment, in particular to a strain of ornithine bacillus which is suitable for treating cyanide-containing wastewater containing thirendan. Background technique [0002] The molecular formula of cartap is C 7 h 15 N 3 o 2 S 2 , White powder with slight odor, soluble in water, slightly soluble in methanol, ethanol, insoluble in acetone, ether, ethyl acetate, chloroform, benzene and other organic solvents. Stable under normal temperature and acidic conditions, unstable under alkaline conditions. No carcinogenic, teratogenic, mutagenic effects, toxic to silkworms, low toxicity to birds, safe to spiders and other natural enemies. Cartap is a nereistoxin insecticide, which has the advantages of high efficiency, broad spectrum, low toxicity, and low residue, and has multiple effects such as systemic, stomach poisoning, and contact killing. Cartap can control pests such as rice borer, rice leaf roller, rice...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C02F101/18C02F101/34C02F101/38C12R1/01
CPCC02F3/34C02F2101/18C02F2101/34C02F2101/38C02F2101/40C12N1/20C12N1/205C12R2001/01
Inventor 肖川杨帆梁佳慧燕定君豆建雄姜文向康杰张剑涛张文武
Owner CHONGQING UNIV OF ARTS & SCI
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