Application of lactobacillus plantarum in preparation of composition for relieving chronic inflammation of organism

A technology of Lactobacillus plantarum and chronic inflammation, which is applied in the field of microorganisms, can solve the problems of limited chronic inflammation, differences in the effect of different strains, and obvious differences in individual intestinal flora and physiological state, etc., to achieve chronic inflammation relief, long shelf life, and stability good sex effect

Active Publication Date: 2021-01-08
HANGZHOU WAHAHA TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these strains are dose- and strain-specific, and individual intestinal flora and physiological state are significantly different, and the effects of different strains are different. Therefore, it is still necessary to develop other new probiotic strains that can relieve chronic inflammation
In addition, the types of inflammatory factors that can be regulated by the existing Lactobacillus plantarum are limited. However, in the chronic inflammation caused by different reasons, the types of inflammatory factors that change are different, and different inflammatory factors have different effects on the body. Therefore, it is now Some Lactobacillus plantarum can adapt to limited chronic inflammation
[0004] In the previous research, the applicant isolated a new strain of Lactobacillus plantarum (Lactobacillus plantarum) 1701 from yogurt powder samples collected in Tibet, and conducted research on its application in weight loss, but whether the strain has the effect of alleviating The ability of chronic inflammation and whether it can have more physiological activities need to be further studied

Method used

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  • Application of lactobacillus plantarum in preparation of composition for relieving chronic inflammation of organism
  • Application of lactobacillus plantarum in preparation of composition for relieving chronic inflammation of organism

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The bacterial strain provided by the present invention is identified as belonging to Lactobacillus plantarum (Lactobacillus plantarum), named 1701 strain, and was preserved in the General Microorganism Culture Collection Center of China Microbiology Culture Collection Management Committee on October 23, 2019, and the microorganism preservation number is CGMCC NO.18728.

[0036] The bacterial strain provided by the present invention is isolated by the inventor from yogurt powder sample collected in the village of Shigatse City, Tibet Autonomous Region of my country.

[0037] The 16S rRNA gene sequence of the strain Lactobacillus plantarum 1701 of the present invention is shown in SEQ ID NO:1.

Embodiment 2

[0039] Kunming mice were reared at a temperature of 21±2°C, with a humidity of 30-70%, 12 hours of alternating light, and free access to maintenance feed and water for mice. Kunming mice were reared for 1 week and killed by dislocation of the cervical vertebrae. The spleens of the mice were aseptically taken out and crushed with a sterile glass syringe core. Sterile Hank's solution containing % fetal bovine serum was used to terminate the lysis, centrifuged at 1000 rpm at 4°C for 5 min, and the pellet was resuspended in 5 mL of RPMI-1640 medium containing 10% fetal bovine serum. Stain with trypan blue, count on a hemocytometer, and calculate the number of viable cells and the rate of viable cells. Adjust the cell concentration to 5 x 10 6 cells / mL.

[0040]After the second-generation activation of the bacterial strain Lactobacillus plantarum 1701 and the control commercial strain Lactobacillus rhamnosus GG (LGG), the bacterial concentration was adjusted to 1×10 7 CFU / mL.

...

Embodiment 3

[0046] Prepare spleen lymphocyte suspension according to the preparation method of mouse spleen lymphocyte in embodiment 2, adjust cell concentration to be 5 * 10 6 cells / mL.

[0047] After the second-generation activation of the bacterial strain Lactobacillus plantarum 1701 and the control commercial strain Lactobacillus rhamnosus GG (LGG), the bacterial concentration was adjusted to 1×10 7 CFU / mL.

[0048] The cell suspension was added to a 96-well cell culture plate, and each treatment was repeated 5 times, divided into zero adjustment group (cell culture medium), blank control group (cell culture medium+cell suspension), bacteria treatment group (cell culture medium+ Cell suspension + bacterial suspension 1×10 7 CFU / mL), 37°C 5% CO 2 Incubate for 48 h in the incubator. After the culture, centrifuge at 1500 rpm for 10 min, draw the supernatant, filter with a 0.22 μm filter membrane, and use ELISA kit to measure the content of IL-10 and IL-12.

[0049] The results are...

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Abstract

The invention relates to the field of microorganisms, and discloses application of lactobacillus plantarum in preparation of a composition for relieving chronic inflammation of an organism. The lactobacillus plantarum is named as 1701, is preserved in China General Microbiological Culture Collection Center on October 23, 2019, has a preservation number of CGMCC No.18728, and is named as lactobacillus plantarum based on classification of microbes. The adopted lactobacillus plantarum can adjust the levels of a cell factor IL-12, an anti-inflammatory factor IL-10 and a proinflammatory factor (including IL-1beta, IL-6, TNF-alpha, MCP-1 and NO) at the same time, so that the lactobacillus plantarum can adapt to chronic inflammation caused by different reasons, and diseases caused by the chronicinflammation can be prevented more comprehensively.

Description

technical field [0001] The invention relates to the field of microbes, in particular to the application of a strain of Lactobacillus plantarum in preparing a composition for relieving chronic inflammation in a body. Background technique [0002] In recent years, with the continuous improvement of people's living standards, a series of health problems have emerged, and sub-health problems have become increasingly prominent. The main causes of sub-health are: unreasonable diet, irregular work and rest, lack of exercise, lack of sleep, high psychological pressure, mental tension, long-term bad emotions, etc. Studies have found that different sub-health patients are more or less accompanied by the occurrence of chronic inflammation in the body, accompanied by the production of MCP-1, which promotes the infiltration of macrophages and the subsequent secretion of inflammatory factors (such as IL-10, IL-6 , TNF-α and NO, etc.). Once the macrophages are activated, the activated ma...

Claims

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Application Information

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IPC IPC(8): A61K35/747A61P29/00A23L33/135A23L2/38A23L2/385A23C19/00A23C9/123A23C9/16A23L19/00A23L29/00
CPCA61K35/747A61K9/0053A61P29/00A23L33/135A23L2/382A23L2/385A23L19/00A23C9/1234A23C9/16A23C19/00A23L29/065A23V2002/00A23V2400/169A23V2200/30
Inventor 孙盛俞赟霞陈苏李言郡余腾斐陈作国陈丽娥郑志瑶陈彩玲周晴晴任学良
Owner HANGZHOU WAHAHA TECH
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