Application of lactobacillus plantarum in preparation of composition for relieving chronic inflammation of organism
A technology of Lactobacillus plantarum and chronic inflammation, which is applied in the field of microorganisms, can solve the problems of limited chronic inflammation, differences in the effect of different strains, and obvious differences in individual intestinal flora and physiological state, etc., to achieve chronic inflammation relief, long shelf life, and stability good sex effect
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Embodiment 1
[0035] The bacterial strain provided by the present invention is identified as belonging to Lactobacillus plantarum (Lactobacillus plantarum), named 1701 strain, and was preserved in the General Microorganism Culture Collection Center of China Microbiology Culture Collection Management Committee on October 23, 2019, and the microorganism preservation number is CGMCC NO.18728.
[0036] The bacterial strain provided by the present invention is isolated by the inventor from yogurt powder sample collected in the village of Shigatse City, Tibet Autonomous Region of my country.
[0037] The 16S rRNA gene sequence of the strain Lactobacillus plantarum 1701 of the present invention is shown in SEQ ID NO:1.
Embodiment 2
[0039] Kunming mice were reared at a temperature of 21±2°C, with a humidity of 30-70%, 12 hours of alternating light, and free access to maintenance feed and water for mice. Kunming mice were reared for 1 week and killed by dislocation of the cervical vertebrae. The spleens of the mice were aseptically taken out and crushed with a sterile glass syringe core. Sterile Hank's solution containing % fetal bovine serum was used to terminate the lysis, centrifuged at 1000 rpm at 4°C for 5 min, and the pellet was resuspended in 5 mL of RPMI-1640 medium containing 10% fetal bovine serum. Stain with trypan blue, count on a hemocytometer, and calculate the number of viable cells and the rate of viable cells. Adjust the cell concentration to 5 x 10 6 cells / mL.
[0040]After the second-generation activation of the bacterial strain Lactobacillus plantarum 1701 and the control commercial strain Lactobacillus rhamnosus GG (LGG), the bacterial concentration was adjusted to 1×10 7 CFU / mL.
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Embodiment 3
[0046] Prepare spleen lymphocyte suspension according to the preparation method of mouse spleen lymphocyte in embodiment 2, adjust cell concentration to be 5 * 10 6 cells / mL.
[0047] After the second-generation activation of the bacterial strain Lactobacillus plantarum 1701 and the control commercial strain Lactobacillus rhamnosus GG (LGG), the bacterial concentration was adjusted to 1×10 7 CFU / mL.
[0048] The cell suspension was added to a 96-well cell culture plate, and each treatment was repeated 5 times, divided into zero adjustment group (cell culture medium), blank control group (cell culture medium+cell suspension), bacteria treatment group (cell culture medium+ Cell suspension + bacterial suspension 1×10 7 CFU / mL), 37°C 5% CO 2 Incubate for 48 h in the incubator. After the culture, centrifuge at 1500 rpm for 10 min, draw the supernatant, filter with a 0.22 μm filter membrane, and use ELISA kit to measure the content of IL-10 and IL-12.
[0049] The results are...
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