Sediment tracing method in river and lake system

Abstract

The invention discloses a sediment tracing method in a river and lake system, which is characterized by comprising the following steps of determining a source point location and a confluence point location of a sediment tracing target area; collecting a target bottom mud sample, and inputting the area and the potential sediment into the lake port point position bottom mud; extracting and collecting the DNA of the sediment sample, carrying out PCR amplification and sequencing, and carrying out microorganism classification based on a gene database; optimizing the sequencing data on the basis ofLEfSe analysis; inputting the optimized sequencing data into a biological traceability model for detection to obtain the contribution proportions of the sediment microorganisms flowing into lakes andlakes from all potential rivers to the sediment microorganisms in the target area; carrying out stability analysis on a detection result, and judging a main contribution river for silt input in the target area according to a judgment scheme; and identifying the upstream sediment source categories and areas of rivers by combining a composite fingerprint identification method. The method has the advantages of being easy to operate, wide in application range, accurate in result and high in efficiency, and has the very important significance for treatment, development and protection of the river and lake system.

Description

technical field [0001] The invention relates to a method for tracing the source of sediment in a river-lake system, and belongs to the technical field of gold monitoring and protection for the river-lake system. Background technique [0002] In the river and lake water environment system, sediment is an important carrier of pollutants, which affects the migration and transformation process of pollutants in the water body, and finally affects the ecological environment conditions of the water body. Sediment carries a large amount of nutrients, pollutants, and microorganisms from rivers entering the lake into the lake, which is an important factor that causes damage to the ecological environment of the lake and eutrophication. The ecological environment of the lake is mainly affected by the sediment that flows into the river. When the ecological environment of the river deteriorates, accurately determine the source of the sediment that flows into the lake and the contribution ...

AI Technical Summary

Problems solved by technology

[0003] The current traditional sediment traceability methods, observation methods and erosion monitoring methods are difficult to guarantee the integrity of collected data and observation data in time and space due to human observation factors, lack of experimental conditions and financial constraints.

The newly developed sediment composite fingerprint identification method has been well applied to the traceability of sediment in river areas, but when applied to more complex river and lake systems, it is not only time-consuming and laborious, but also requires a large number of samples of different soil types for each river , and the physical and chemical properties of the sediment input by each river are not highly differentiated, resulting in the inability of such fingerprint factors to accurately predict the source of sediment in the lake area in the river-lake system

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