Paracoccus thiooxidans capable of efficiently degrading DMF (Dimethyl Formamide) and application of Paracoccus thiooxidans in treatment of DMF-containing wastewater

A technology of sulfur oxide and paracoccus, applied in biological water/sewage treatment, water/sludge/sewage treatment, bacteria, etc., can solve the problem of less research, and achieve the effect of high DMF tolerance, high efficiency and stable application

Pending Publication Date: 2022-05-10
浙江台州秀川科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] Paracoccus sulfuroxidans is a new species discovered and named by Liu et al. in 2006 (Liu X Y, Wang B J, Jiang C Y, et al. Paracoccus sulfuroxidans sp.nov.asulfur oxidizer from activated sludge[J].Int J Syst Evol Microbiol, 2006,56 (11): 2693-2695.), there are few studies about this bacterial species at present, and there is no literature report that the bacterial strain in Paracoccus sulfur peroxide has the ability to degrade DMF

Method used

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  • Paracoccus thiooxidans capable of efficiently degrading DMF (Dimethyl Formamide) and application of Paracoccus thiooxidans in treatment of DMF-containing wastewater
  • Paracoccus thiooxidans capable of efficiently degrading DMF (Dimethyl Formamide) and application of Paracoccus thiooxidans in treatment of DMF-containing wastewater
  • Paracoccus thiooxidans capable of efficiently degrading DMF (Dimethyl Formamide) and application of Paracoccus thiooxidans in treatment of DMF-containing wastewater

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1: preparation culture medium

[0048] Three media were prepared according to the following recipes:

[0049] Inorganic salt medium (g / L): KH 2 PO 4 1.2, K 2 HPO 4 6.8, NaCl 0.5, MgSO 4 ·7H 2 O 0.1, MnSO 4 ·H 2 O 0.1, CaCl 2 0.1, FeSO 4 ·7H 2 O 0.1, Na 2 MoO 4 2H 2 O 0.006, CuSO 4 ·5H 2 O 0.006, ZnSO 4 ·7H 2 O 0.007, CoCl 2 ·6H 2 O 0.0001, H 3 BO 3 0.0124, vitamin B1 0.00001, with DMF as the sole carbon source and nitrogen source, pH=6 (add 2% agar powder by mass fraction to the solid medium).

[0050] LB medium (g / L): peptone 10.0, yeast extract 5.0, NaCl 10.0, pH=7 (solid medium with 2% mass fraction of agar powder added).

[0051] DMF1 medium: Add 0.2g / L DMF and 0.2g / L dimethylamine to LB medium.

Embodiment 2

[0052] Embodiment 2: Strain domestication, isolation and identification

[0053] (1) Strain domestication:

[0054] Inoculate 10 mL of fresh sludge from each pharmaceutical factory into a Erlenmeyer flask filled with 100 mL of inorganic salt medium (DMF concentration: 1000 mg / L), and place it in a shaking incubator at 30 °C and 130 r / min for 1 week. Take samples every other day to detect TN and NH in the system 3 -N value. After 1 week of culture, the NH in the system 3 -N obviously increased to about 160mg / L, and the ammoniation rate of TN reached over 90%. Transfer to fresh inorganic salt culture medium (DMF concentration is 2000mg / L) according to 10% inoculum amount again, monitor the TN and NH in the system 3 -N value, continue culturing for 1 week. Continuous transfer was performed 5 times, and the DMF concentration was gradually increased to 5000 mg / L.

[0055] Note: Ammonification refers to the conversion of organic nitrogen in TN into NH 3 -N.

[0056] (2) Stra...

Embodiment 3

[0064] Embodiment 3: single bacterium degrades DMF ability

[0065] (1) Seed liquid preparation:

[0066] The 4 strains P.sulfuroxidans 175A1-1, Aquamicrobiumlusatiense 3-1-1, Gelidibacter flavus 2-CZ-5, and Sphingomonas paucimobilis 3-3-10 isolated in Example 2 were amplified and cultured in LB medium to OD 600 All reached about 1.5, centrifuged at 6000r / min for 3min, discarded the supernatant, and took the bottom bacteria. Then resuspend with sterile distilled water to prepare seed solution.

[0067] (2) Experimental device:

[0068] The experimental device is a continuous water inlet and outlet device, which simulates the on-site treatment system to the greatest extent to test the treatment effect of bacteria on DMF. The complete set such as Figure 5 As shown, it includes the water inlet pool, the strain treatment pool and the water outlet pool connected in sequence. The effective volume of each pool is 3L, the water inlet pool is used to add nutrients to adjust the ...

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Abstract

The invention relates to the technical field of DMF (Dimethyl Formamide) degradation, and provides paracoccus thiooxidans capable of efficiently degrading DMF and application of the paracoccus thiooxidans in treatment of DMF-containing wastewater. The paracoccus thiooxidans is named as 175A1-1, has been preserved in the China General Microbiological Culture Collection Center on October 25, 2021, the preservation number of the paracoccus thiooxidans is CGMCC NO.23659, and the classification of the microorganisms is named as paracoccus thiooxidans. The paracoccus thiooxidans 175A1-1 disclosed by the invention has relatively high DMF (Dimethyl Formamide) tolerance and can be used for converting DMF into NH3-N by taking DMF as a unique carbon source and a unique nitrogen source, so that efficient ammonification and mineralization of DMF are realized; in addition, the strain can be efficiently and stably applied to field engineering.

Description

technical field [0001] The invention relates to the technical field of DMF degradation, in particular to a strain of Paracoccus sulfoxidans capable of efficiently degrading DMF and its application in the treatment of DMF-containing wastewater. Background technique [0002] N,N-dimethylformamide (N,N-dimethylformamide, DMF) is an organic solvent with stable chemical properties, high boiling point and excellent performance. In addition to halogenated hydrocarbons, it can be miscible with water and most organic solvents in any proportion. Because of its good solubility, it is called "universal solvent" and is also widely used in synthetic textiles, leather, electronics, pharmaceuticals and pesticides. . Therefore, DMF is also one of the common high-concentration pollutants in industrial wastewater. [0003] DMF wastewater treatment methods include physical methods (such as adsorption method, membrane method), chemical methods (such as photocatalytic oxidation, plasma oxidatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/34C02F101/38
CPCC12N1/20C02F3/34C02F2101/38C02F2101/34
Inventor 苏悦张文武杨宇斯夏雨王开彬李书峰孙聪徐林
Owner 浙江台州秀川科技有限公司
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