Bacillus amyloliquefaciens strain and its application in waste deodorization

A technology for dissolving starch spores and bacilli, applied in the directions of bacteria, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of low removal rate of malodorous substances, achieve good deodorization effect, wide application prospects, and simple cultivation operation. Effect

Inactive Publication Date: 2017-01-04
NANJING RUNZHONG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Solve the technical problem of low removal rate of malodorous substances in the existing garbage deodorization

Method used

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  • Bacillus amyloliquefaciens strain and its application in waste deodorization
  • Bacillus amyloliquefaciens strain and its application in waste deodorization
  • Bacillus amyloliquefaciens strain and its application in waste deodorization

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A strain of Bacillus amyloliquefaciens in Example 1, which is Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) YZ-0001, was preserved in the General Microbiology Center of China Committee for the Preservation of Microorganisms, with a preservation number of CGMCC 12052 and a preservation date of 2016. January 14th.

[0026] The Bacillus amyloliquefaciens strain is isolated from the soil, and the characteristics of the colonies shown on the screening plate are: round shape, smooth surface, dirty white, and Gram staining is positive.

[0027] Specifically, the isolation and identification process of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) YZ-0001 strain is as follows:

[0028] Weigh 10 g of the collected soil, add it to a Erlenmeyer flask filled with 90 mL of sterile normal saline, shake it on a constant temperature shaker at 28 ° C at 150 r / min for 10 minutes, let it stand for 1 minute, and transfer 1 mL of the supernatant to a 9 mL sterile In nor...

Embodiment 2

[0030] Example 2 is the application of Bacillus amyloliquefaciens YZ-0001 in garbage deodorization.

[0031] Preparation of microbial deodorant: Inoculate the Bacillus amyloliquefaciens YZ-0001 of Example 1 into the enrichment medium, and cultivate it with shaking at 30°C until the logarithmic growth phase (bacteria concentration is about 106-1010 / ml). Using water as the solute, according to the mass concentration, mix 1% to 2% of the stock solution of the strain, 4% to 6% of the nutrient and water, and ferment for 48 hours at a temperature of 20 to 25°C to obtain the microbial detoxification agent. odorant. Among them, the preparation method of the nutritional agent is: 200 grams of sugar, 50 grams of potassium dihydrogen phosphate, 30 grams of peptone, 20 grams of magnesium sulfate, and 10,000 milliliters of water. Add to warm water, and stir until the raw materials are completely dissolved in water to obtain a nutritional supplement.

[0032] The application effect test o...

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Abstract

The invention provides a bacillus amyloliquefaciens strain and its application in waste deodorization. The stratin is bacillus amyloliquefaciens YZ-0001, and collected at the China General Microbiological Culture Collection Center; the collection number is CGCC 12052 and the collection date is January 14, 2016. The bacillus amyloliquefaciens strain is isolated from soil; the colony characteristics shown on a screening flat panel (LB flat panel) is: the form is round, the surface is smooth and dirty white, and the gram stain is positive. Through the 16Sr DNA microorganism classification and identification, it is determined as bacillus amyloliquefaciens. The bacillus amyloliquefaciens strain has strong and complex organic matter degradation ability; therefore, it can be applied to the waste deodorization, and the deodorization effect is good.

Description

technical field [0001] The invention relates to the technical field of bacillus, in particular to a strain of bacillus amyloliquefaciens and its application in garbage deodorization. Background technique [0002] The annual output of urban garbage in my country is nearly 150 million tons, and it is increasing at a rate of about 8% every year. Nearly 2 / 3 of the cities are trapped in the predicament of garbage siege. So far, garbage in many cities is still piled up in exposed locations on the outskirts of the city without any protective measures. Mosquitoes and flies are born, rats are infested, and the smell is everywhere. Large pollution, seriously endangering human health. [0003] The smell of garbage is caused by the decomposition of organic components in garbage by bacteria. 75% to 80% of domestic garbage is organic matter, mainly including fruit peels, vegetable leaves and stalks, leftovers, leftovers, poultry, animals and fish. Skin, fur, viscera, fat, feces, leftove...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B01D53/84C12R1/07
CPCB01D53/84C12N1/20C12N1/205C12R2001/07Y02A50/20
Inventor 管玉柱陈家庆吴山功
Owner NANJING RUNZHONG BIOTECH
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