Bacillus amyloliquefaciens strain and its application in waste deodorization
A technology for dissolving starch spores and bacilli, applied in the directions of bacteria, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of low removal rate of malodorous substances, achieve good deodorization effect, wide application prospects, and simple cultivation operation. Effect
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Embodiment 1
[0025] A strain of Bacillus amyloliquefaciens in Example 1, which is Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) YZ-0001, was preserved in the General Microbiology Center of China Committee for the Preservation of Microorganisms, with a preservation number of CGMCC 12052 and a preservation date of 2016. January 14th.
[0026] The Bacillus amyloliquefaciens strain is isolated from the soil, and the characteristics of the colonies shown on the screening plate are: round shape, smooth surface, dirty white, and Gram staining is positive.
[0027] Specifically, the isolation and identification process of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) YZ-0001 strain is as follows:
[0028] Weigh 10 g of the collected soil, add it to a Erlenmeyer flask filled with 90 mL of sterile normal saline, shake it on a constant temperature shaker at 28 ° C at 150 r / min for 10 minutes, let it stand for 1 minute, and transfer 1 mL of the supernatant to a 9 mL sterile In nor...
Embodiment 2
[0030] Example 2 is the application of Bacillus amyloliquefaciens YZ-0001 in garbage deodorization.
[0031] Preparation of microbial deodorant: Inoculate the Bacillus amyloliquefaciens YZ-0001 of Example 1 into the enrichment medium, and cultivate it with shaking at 30°C until the logarithmic growth phase (bacteria concentration is about 106-1010 / ml). Using water as the solute, according to the mass concentration, mix 1% to 2% of the stock solution of the strain, 4% to 6% of the nutrient and water, and ferment for 48 hours at a temperature of 20 to 25°C to obtain the microbial detoxification agent. odorant. Among them, the preparation method of the nutritional agent is: 200 grams of sugar, 50 grams of potassium dihydrogen phosphate, 30 grams of peptone, 20 grams of magnesium sulfate, and 10,000 milliliters of water. Add to warm water, and stir until the raw materials are completely dissolved in water to obtain a nutritional supplement.
[0032] The application effect test o...
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