Isolation, identification and application of a high prodigiosin-producing marine bacterium
A technology of prodigiosin and marine bacteria, applied in the field of microorganisms, can solve the problems of difficult fermentation and production, low prodigiosin production, etc., and achieve the effects of easy expansion of cultivation, high purity, and stable yield
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Embodiment 1
[0024] 1. Preservation information:
[0025] This example provides a strain of marine bacteria IMDGX0109, the strain has been preserved, preservation number: CCTCC NO: M2018148; preservation place: China, Wuhan, Wuhan University, China Center for Typical Culture Collection; preservation date: March 22, 2018 .
[0026] 2. Source and purification preservation:
[0027] The strain is obtained by separating and purifying from the rough shore snail in the Beibu Gulf or the sponge Pseudoceratina sp. collected from Weizhou Island in the North Sea. The specific process of separation and purification is as follows:
[0028] (1) Rinse the surface of the rough shore snail collected from Beihai Beach, Guangxi with sterile water three times, disinfect the surface with ethanol with a mass fraction of 75%, incise and take out the snail meat, and cut the tissue with a sterile scalpel Crushed, weighed 1g, put it into a grinder to grind it fully, added 2mL sterile water and mixed with the sam...
Embodiment 2
[0056] The preparation steps are basically the same as in Example 1, except that the fermentation conditions in the step (1) of the preparation step of the fermentation product are a temperature of 28° C., a rotation speed of 180 rpm / min, and a culture time of 23 hours to obtain a fermentation broth.
[0057] The yield after detection and purification reaches 0.656g / L.
Embodiment 3
[0059] The preparation steps are basically the same as those in Example 1, except that the fermentation conditions in step (1) of the preparation step of the fermentation product are a temperature of 29° C., a rotation speed of 190 rpm / min, and a culture time of 28 hours to obtain a fermentation broth.
[0060] The yield after detection and purification reaches 0.635g / L.
[0061] Control group 1:
[0062] The same preparation method as in Example 1, the difference is that the activation medium in the preparation of the fermentation product is the medium of ISP 2: 4.0g of yeast extract, 10.0g of wort juice, 4.0g of glucose, 20.0g of agar, 1000ml of distilled water, pH 7.0.
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