Isolation, identification and application of a high prodigiosin-producing marine bacterium

A technology of prodigiosin and marine bacteria, applied in the field of microorganisms, can solve the problems of difficult fermentation and production, low prodigiosin production, etc., and achieve the effects of easy expansion of cultivation, high purity, and stable yield

Active Publication Date: 2022-02-01
广西默士药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Serratia is not an excellent production strain, and is an conditional pathogen. The production of prodigiosin is relatively low and affected by separation and purification technology, so it is difficult to achieve large-scale industrial fermentation and production

Method used

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  • Isolation, identification and application of a high prodigiosin-producing marine bacterium
  • Isolation, identification and application of a high prodigiosin-producing marine bacterium
  • Isolation, identification and application of a high prodigiosin-producing marine bacterium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Preservation information:

[0025] This example provides a strain of marine bacteria IMDGX0109, the strain has been preserved, preservation number: CCTCC NO: M2018148; preservation place: China, Wuhan, Wuhan University, China Center for Typical Culture Collection; preservation date: March 22, 2018 .

[0026] 2. Source and purification preservation:

[0027] The strain is obtained by separating and purifying from the rough shore snail in the Beibu Gulf or the sponge Pseudoceratina sp. collected from Weizhou Island in the North Sea. The specific process of separation and purification is as follows:

[0028] (1) Rinse the surface of the rough shore snail collected from Beihai Beach, Guangxi with sterile water three times, disinfect the surface with ethanol with a mass fraction of 75%, incise and take out the snail meat, and cut the tissue with a sterile scalpel Crushed, weighed 1g, put it into a grinder to grind it fully, added 2mL sterile water and mixed with the sam...

Embodiment 2

[0056] The preparation steps are basically the same as in Example 1, except that the fermentation conditions in the step (1) of the preparation step of the fermentation product are a temperature of 28° C., a rotation speed of 180 rpm / min, and a culture time of 23 hours to obtain a fermentation broth.

[0057] The yield after detection and purification reaches 0.656g / L.

Embodiment 3

[0059] The preparation steps are basically the same as those in Example 1, except that the fermentation conditions in step (1) of the preparation step of the fermentation product are a temperature of 29° C., a rotation speed of 190 rpm / min, and a culture time of 28 hours to obtain a fermentation broth.

[0060] The yield after detection and purification reaches 0.635g / L.

[0061] Control group 1:

[0062] The same preparation method as in Example 1, the difference is that the activation medium in the preparation of the fermentation product is the medium of ISP 2: 4.0g of yeast extract, 10.0g of wort juice, 4.0g of glucose, 20.0g of agar, 1000ml of distilled water, pH 7.0.

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Abstract

The invention relates to the technical field of microorganisms, in particular to the isolation and identification of a strain of marine bacteria and its application. The marine bacterium is named Notoacmeibacter marinus IMDGXO 109, and the preservation number is CCTCC NO: M 2018148; the preservation place is: China, Wuhan, Wuhan University, China Center for Type Culture Collection, and the preservation time is March 22, 2018. Through the operation steps of the present application, the yield and stability of the target product prodigiosin of the strain can be improved, and the produced metabolite has high purity and good safety, and can be applied in the pharmaceutical industry.

Description

【Technical field】 [0001] The invention relates to the technical field of microorganisms, in particular to the isolation, identification and application of a high-prodigiosin-producing marine bacterium. 【Background technique】 [0002] The secondary metabolites produced by microorganisms are not necessary for their growth and reproduction (pigments, antibiotics, toxins, and hormones, etc.), but they have very important research value and significance for human activities. Prodigiosin widely exists in nature and has various biological activities such as antibacterial, antitumor, antimalarial and immunosuppressive. Therefore, it has good application value and market prospect in the pharmaceutical industry. [0003] At present, there are few reports on the screening of new strains of prodigiosin-producing microorganisms and new strains of marine bacteria at home and abroad. The research on prodigiosin at home and abroad mainly focuses on the optimization of the fermentation proc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/02C12P17/16C12R1/01
CPCC12N1/02C12N1/20C12P17/165C12R2001/01C12N1/205
Inventor 高程海米顺利丁永奎苏志维杜瑜欣易湘茜
Owner 广西默士药业有限公司
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