High-yield prodigiosin marine bacteria separation identification and application

A technology of prodigiosin and marine bacteria, applied in the field of microorganisms, can solve the problems of difficulty in fermentation and production, low yield of prodigiosin, etc., achieve important market value and significance, high purity, and short production cycle

Active Publication Date: 2018-10-26
GUANGXI UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Serratia is not an excellent production strain, and is an conditional pathogen. The production of prodigiosin is relatively low and affected by separation and purification technology, so it is difficult to achieve large-scale industrial fermentation and production

Method used

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  • High-yield prodigiosin marine bacteria separation identification and application
  • High-yield prodigiosin marine bacteria separation identification and application
  • High-yield prodigiosin marine bacteria separation identification and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Preservation information:

[0025] This example provides a strain of marine bacteria IMDGX0109, the strain has been preserved, preservation number: CCTCC NO: M2018148; preservation place: China, Wuhan, Wuhan University, China Center for Typical Culture Collection; preservation date: March 22, 2018 .

[0026] 2. Source and purification preservation:

[0027] The strain is obtained by separating and purifying from the rough shore snail in the Beibu Gulf or the sponge Pseudoceratina sp. collected from Weizhou Island in the North Sea. The specific process of separation and purification is as follows:

[0028] (1) Rinse the surface of the rough shore snail collected from Beihai Beach, Guangxi with sterile water three times, disinfect the surface with ethanol with a mass fraction of 75%, incise and take out the snail meat, and cut the tissue with a sterile scalpel Crushed, weighed 1g, put it into a grinder to grind it fully, added 2mL sterile water and mixed with the sam...

Embodiment 2

[0056] The preparation steps are basically the same as in Example 1, except that the fermentation conditions in the step (1) of the preparation step of the fermentation product are a temperature of 28° C., a rotation speed of 180 rpm / min, and a culture time of 23 hours to obtain a fermentation broth.

[0057] The yield after detection and purification reaches 0.656g / L.

Embodiment 3

[0059] The preparation steps are basically the same as those in Example 1, except that the fermentation conditions in step (1) of the preparation step of the fermentation product are a temperature of 29° C., a rotation speed of 190 rpm / min, and a culture time of 28 hours to obtain a fermentation broth.

[0060] The yield after detection and purification reaches 0.635g / L.

[0061] Control group 1:

[0062] The same preparation method as in Example 1, the difference is that the activation medium in the preparation of the fermentation product is the medium of ISP 2: 4.0g of yeast extract, 10.0g of wort juice, 4.0g of glucose, 20.0g of agar, 1000ml of distilled water, pH 7.0.

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Abstract

The invention relates to the technical field of microorganisms, in particular to ocean bacteria separation identification and application thereof. The ocean bacterium is named as Notoacmeibacter marinus IMDGXO 109, the preservation number is CCTCC NO: M 2018148; and the preservation site is China Center for Type Culture Collection in Wuhan University, Wuhan China, and the preservation time was onMarch 22, 2018. By virtue of the operation steps of the invention, the yield and stability of the target product prodigiosin of the strain can be improved, and the produced metabolism product is highin purity and good in safety and can be applied to the pharmaceutical industry.

Description

【Technical field】 [0001] The invention relates to the technical field of microorganisms, in particular to the isolation, identification and application of a high-prodigiosin-producing marine bacterium. 【Background technique】 [0002] The secondary metabolites produced by microorganisms are not necessary for their growth and reproduction (pigments, antibiotics, toxins, and hormones, etc.), but they have very important research value and significance for human activities. Prodigiosin widely exists in nature and has various biological activities such as antibacterial, antitumor, antimalarial and immunosuppressive. Therefore, it has good application value and market prospect in the pharmaceutical industry. [0003] At present, there are few reports on the screening of new strains of prodigiosin-producing microorganisms and new strains of marine bacteria at home and abroad. The research on prodigiosin at home and abroad mainly focuses on the optimization of the fermentation proc...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02C12P17/16C12R1/01
CPCC12N1/02C12N1/20C12P17/165C12R2001/01C12N1/205
Inventor 高程海米顺利丁永奎苏志维杜瑜欣易湘茜
Owner GUANGXI UNIV OF CHINESE MEDICINE
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