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High-temperature-resistant bacillus hisashii and high-temperature compost fermentation method

A technology of bacillus and fermentation method, which is applied in the field of microbial technology and environmental engineering, and can solve the problems of difficulty in reaching higher fermentation temperature, lack of fermentation, and death of functional microorganisms.

Active Publication Date: 2020-03-06
浙江佳河绿环境科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional composting practices believe that the composting fermentation temperature should not be higher than 70°C, otherwise functional microorganisms will die in large numbers, and the fermentation process will slow down, resulting in incomplete fermentation
[0004] In recent years, studies have shown that increasing the temperature of the heap can accelerate the humification process and shorten the fermentation period. Thermophilic functional microorganisms are the key factor determining the fermentation temperature. Traditional high-temperature compost lacks extreme thermophilic functional microorganisms, making it difficult to achieve higher fermentation temperatures.

Method used

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  • High-temperature-resistant bacillus hisashii and high-temperature compost fermentation method

Examples

Experimental program
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Effect test

Embodiment 1

[0060] Embodiment 1: Food waste composting treatment

[0061] Take 5 tons of kitchen waste, add it to the fermentation device with cutting and stirring, and continuously cut and stir it into pieces of about 5cm, put in 3% quicklime, control the moisture content of the material at 80%, put in 2.5g of alkaline amylase after mixing , 1g of alkaline protease and 2.5g of esterase continued to mix and stir, and turned on the electric heating to raise the temperature to 35°C and maintain it for 1h. Put in 2 tons of HN-1 inoculant sawdust, (during production, the volume ratio of liquid inoculant to dry sawdust is 1:1.1) and stir thoroughly, increase the electric heating power to raise the temperature to 45°C, and start high-temperature composting. After 4 hours of start-up, the temperature of the compost heap rose to 65°C, and gradually increased within 24 hours of start-up, and finally reached 85-100°C, and the humidity gradually decreased from the initial 80% to about 50% at last. ...

Embodiment 2

[0062] Example 2: Farming solid waste composting

[0063] Take 10 tons of pig farm feces, put in 4% quicklime, stir evenly, control the moisture content of the material at 82%, put in 5.5g of alkaline amylase, 5.0g of alkaline protease, and 10g of esterase, continue to mix and stir, and turn on the electric heating to raise the temperature to 40°C and maintain for 1h. Put in 4 tons of HN-1 inoculant sawdust, (during production, the volume ratio of liquid inoculum to dry sawdust is 1:0.8) and stir thoroughly, increase the electric heating power to raise the temperature to 45°C, and start high-temperature composting. 4 hours after start-up, the temperature of the compost heap rose to 65°C, and gradually increased within 24 hours of start-up, and finally reached 85-100°C, and the humidity gradually decreased from the initial 80% to about 55% at last. Then the temperature dropped slowly, and the temperature dropped to 65°C within 48 hours of starting. At this time, 20% of the vol...

Embodiment 3

[0064] Example 3: Fertilizer composting of agricultural by-products

[0065] Take 5 tons of straw, add it to the fermentation device with cutting and stirring, and continuously cut and stir it into pieces of about 6cm. Put in 3% quicklime, stir evenly, control the moisture content of the material at 85%, put in 3.0g alkaline amylase, 2.5g alkali Protease and 4.0 g of esterase were mixed and stirred, and the electric heating was turned on to raise the temperature to 40° C. and maintained for 2 hours. Put in 2.5 tons of HN-1 inoculum sawdust, (during production, the volume ratio of liquid inoculum to dry sawdust is 1:1.2) and stir thoroughly, increase the electric heating power to raise the temperature to 45°C, and start high-temperature composting. Six hours after start-up, the temperature of the compost heap rose to 65°C, and gradually increased within 24 hours of start-up, and finally reached 85-100°C, and the humidity gradually decreased from the initial 85% to about 55% at ...

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Abstract

The invention, which relates to the fields of microbial technology and environmental engineering, discloses high-temperature-resistant bacillus hisashii and a high-temperature compost fermentation method. The high-temperature-resistant bacillus hisashii is named as NH-1 and the preservation number of the high-temperature-resistant bacillus hisashii is CGMCC 17919; and the microorganism is classified and named as bacillus hisashii. According to the invention, the strain can normally metabolize and grow at a temperature of 35 to 100 DEG C under the conditions of the pH value being 6 to 11 and the salinity being 0 to 5%; growth can be realized by utilizing various carbon sources and nitrogen sources; and the substrate spectrum is wide. The bacterial strain can be used for carrying out high-temperature aerobic composting treatment on various organic matters such as kitchen wastes, agricultural product tailings, slaughter house wastes in cooperation with the special high-temperature composting process, so that the organic matter materials are processed by humification and dehydration drying ad the weight is reduced by 65 to 90% and thus an organic fertilizer with excellent quality is formed.

Description

technical field [0001] The invention relates to the fields of microbial technology and environmental engineering, in particular to a high-temperature resistant Bacillus Miyamoto and a high-temperature composting fermentation method. Background technique [0002] High-temperature composting is a procedural noun, specifically a method of waste disposal. High-temperature composting technology is to use microorganisms to biodegrade organic matter that can be decomposed in waste under certain external conditions, making it a similar A process based on fertile humus soil. This high-temperature composting method has certain characteristics for waste, and organic waste is a prerequisite for the survival and reproduction of microorganisms. According to the preferences and living habits of different microorganisms, high-temperature composting is divided into aerobic high-temperature composting technology and anaerobic high-temperature composting technology. Among them, aerobic high-...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C05F15/00C05F17/00C05F17/20C12R1/07
CPCC02F3/34C05F9/00C05F17/00C12N1/205C12R2001/07Y02W30/40
Inventor 张文武何建鹏郑多日林进龙
Owner 浙江佳河绿环境科技有限公司
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