Pichia pastoris strain for producing acid protease

An acid protease and yeast strain technology, applied in the field of microorganisms, can solve the problems of lack of fermentation strains and lag in fermentation process research.

Pending Publication Date: 2020-06-16
OCEAN RES CENT OF ZHOUSHAN ZHEJIANG UNIV
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Problems solved by technology

Generally speaking, the main problem of the current preparation of fish protein fertilizer is: the research on fermentation process is seriously lagging behind, especially the extreme shortage of efficient fermentation strains

Method used

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  • Pichia pastoris strain for producing acid protease
  • Pichia pastoris strain for producing acid protease

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Experimental program
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specific Embodiment

[0029] (1) Fermentation test in 7.5L fermenter

[0030] Under the condition of aeration rate of 4L / min and 400r / min, transfer fermentation with 3% inoculum size for 24h, medium formula is 50g / L of waste molasses, 50g / L of soybean meal, NaH 2 PO 4 11.55g / L; Magnesium sulfate 1.14g / L; The salinity of the medium is 22.76; The pH is adjusted to 3-4. In the end, the enzyme activity of the crude enzyme solution reached 9854.4±4.5U / mL. In order to obtain a better development effect of amino acid liquid fertilizer, the enzyme activity of the crude enzyme solution was further studied. g / L manganese ions can make the protease activity of crude enzyme solution reach 10542.9±2.7U / mL.

[0031] (2) Development of amino acid liquid fertilizer: Based on (1), carry out preliminary research on amino acid liquid fertilizer, according to fish soluble slurry 50g / L, waste molasses 50g / L, NaH 2 PO 4 11.55g / L; the proportion of magnesium sulfate 1.14g / L is used to prepare raw materials; then th...

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Abstract

The present invention relates to the field of microorganisms, and discloses a pichia pastoris strain for producing acid protease. The pichia pastoris strain is named as ZJU-c, is preserved in China General Microbiological Culture Collection Center on October 12, 2018, the preservation number of the Pichia pastoris strain is CGMCC 16580, and the Pichia pastoris strain is classified and named as Pichia pastoris. Compared with other similar strains, the pichia pastoris strain disclosed by the invention has the characteristic of high yield of acid protease; the pichia pastoris strain disclosed bythe invention is applied to development of amino acid liquid fertilizer, and the content of amino acid in obtained fermentation liquor is higher.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to an acid protease-producing Pichia pastoris strain. Background technique [0002] During the processing of aquatic products, the usable part only accounts for 50-70%, and about 30% becomes by-products, which include fish heads, fish steaks (bone), minced meat, viscera, fish tails, fish skins, etc. Contain protein, fat, amino acid and other nutrients. Among the waste protein leftovers, fish lysate is a common waste. Among them, fish soluble paste is a large amount of water-soluble substances that are separated into liquids after cooking and pressing during the fish meal production process. After research, it was found that fish soluble paste contains a large number of beneficial ingredients such as small molecular peptides, amino acids, taurine, and minerals, and retains the unique unknown growth factors of fish meal. If these waste proteins can be used for high-value resource util...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12N9/58C05C11/00C05F17/20C12R1/84
CPCC12N9/58C12N1/16C05C11/00C12R2001/84C12N1/165Y02W30/40
Inventor 郑刚杨志坚肖金星王开扬泮红文陈磊
Owner OCEAN RES CENT OF ZHOUSHAN ZHEJIANG UNIV
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