Reagent card for quantitatively detecting helicobacter pylori antibody by fluorescence chromatography and detection method

A technology of Helicobacter pylori and fluorescence chromatography, which is applied in biological testing, measuring devices, immunoassays, etc., can solve the problems of difficult research and development, and achieve the effect of reasonable design and quick and convenient use

Inactive Publication Date: 2020-12-04
三门县人民医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fluorescence chromatography detection antibody methods currently include indirect method and double-antigen sandwich method. The indirect method only needs one antigen, which is relatively simple to develop, but it is difficult to maintain a good level of sensitivity and specificity at the same time, and some false positives and False negative results; the double-antigen sandwich method requires paired antigens, which is more difficult to develop, but the detection sensitivity and specificity are significantly higher than the indirect method
At present, there is no lanthanide time-resolved fluorescence chromatography double-antigen sandwich detection reagent that can quantitatively detect Helicobacter pylori antibodies.

Method used

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  • Reagent card for quantitatively detecting helicobacter pylori antibody by fluorescence chromatography and detection method
  • Reagent card for quantitatively detecting helicobacter pylori antibody by fluorescence chromatography and detection method

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Embodiment

[0024] The reagent card for the quantitative detection of Helicobacter pylori antibody by fluorescence chromatography in this embodiment was prepared according to the following steps.

[0025]1. Helicobacter pylori recombinant protein antigen-coated latex microspheres labeled with lanthanide elements: the particle size of the latex microspheres is 100nm, and the Helicobacter pylori recombinant protein antigen is coated with lanthanide-labeled latex microspheres Ball 6h, coating concentration is 30μg Helicobacter pylori recombinant protein antigen / 1mg latex microsphere labeled with lanthanide, centrifuged under the condition of 14000r / min for 30min, isolate the latex microsphere coated Helicobacter pylori recombinant protein antigen, and washed 3 times with 0.01mol / L PBS, and then added to a final concentration of 1-2% BSA and 1-2% casein to seal the latex microspheres coated with Helicobacter pylori recombinant protein antigen for 30 minutes; Wash the latex microspheres of the...

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Abstract

The reagent card for quantitatively detecting the helicobacter pylori antibody by fluorescence chromatography comprises a card shell and a test strip arranged in the card shell, wherein the test stripcomprises a bottom plate, and a nitrocellulose membrane, a conjugate pad and a sample pad which are adhered to the bottom plate; the sample pad, the conjugate pad and the nitrocellulose membrane aresequentially overlapped end to end for a preset length; the nitrocellulose membrane is coated with a helicobacter pylori natural antigen through lineation; and lanthanide-labeled latex microspheres coated with helicobacter pylori recombinant protein antigens are sprayed on the conjugate pad. The reagent card provided by the invention is a helicobacter pylori antibody lanthanide time-resolved fluorescence chromatography quantitative detection reagent card prepared by adopting a helicobacter pylori recombinant protein antigen and natural antigen combined double-antigen sandwich method, and can realize rapid quantitative detection of a helicobacter pylori antibody (IgG antibody). Correspondingly, the invention further provides a detection method for quantitatively detecting the helicobacter pylori antibody through fluorescence chromatography.

Description

technical field [0001] The invention relates to a detection technology of Helicobacter pylori antibody, in particular to a reagent card and a detection method for the quantitative detection of Helicobacter pylori antibody by fluorescence chromatography. Background technique [0002] Helicobacter pylori (H.p) was first discovered by Barry J.Marshall and J.Robin Warren, who won the 2005 Nobel Prize in Physiology or Medicine. Helicobacter pylori is a unipolar, multi-flagellate, blunt-ended, spirally curved bacterium, 2.5-4.0 μm long and 0.5-1.0 μm wide. On the surface of gastric mucosal epithelial cells, it often presents a typical spiral or arc shape. [0003] Helicobacter pylori infection is the main pathogenic factor of chronic active gastritis, peptic ulcer and gastric mucosa-associated lymphoid tissue lymphoma, and is closely related to the occurrence of gastric cancer. In 1994, the World Health Organization / International Agency for Research on Cancer (WHO / IARC) classifi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/558G01N33/543G01N33/533G01N33/68
CPCG01N33/56911G01N33/558G01N33/54313G01N33/533G01N33/6854G01N2333/195G01N2469/20
Inventor 黎宏章赵俊
Owner 三门县人民医院
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