A kind of heparin sodium production equipment based on the secondary enzymatic hydrolysis method

Abstract

The invention discloses a heparin sodium production equipment based on a secondary enzymatic hydrolysis method, which includes a cylinder, a foot, a stirring device, a cover plate, a first rotating part, and a detection structure; the stirring device includes a stirring device set in the cylinder cavity, the first cavity set on the inner wall of the cylinder, the heating tube set in the first cavity, the motor set on the cover plate, the telescopic stirring structure set under the cover plate, the The first auxiliary structure on the side wall of the stirring chamber and the discharge structure at the bottom of the stirring chamber; through the setting of the telescopic stirring structure in the present invention, the stirring shaft can be contracted after the stirring is completed and opened in the form of a flip cover , so that the design height of the tank body is higher, thereby reducing the occupied area, increasing the storage volume of the equipment, and making it more convenient for internal cleaning; at the same time, the heating efficiency is effectively guaranteed by the side wall heating method, and the internal The temperature is balanced and the preparation efficiency is improved at the same time.

Description

technical field [0001] The invention belongs to the technical field of biotechnology, and in particular relates to a production equipment for heparin sodium based on a secondary enzymatic hydrolysis method. Background technique [0002] In the process of preparing heparin sodium, add the prepared porcine intestinal mucosa raw material into the sodium chloride aqueous solution with a salinity of 4.0 to 4.5 degrees, mix evenly to obtain a mixed solution, and add a pH regulator to adjust the pH of the mixed solution. pH value, and the temperature of the mixture was raised to 33±2°C, then alkaline protease was added, the temperature was continuously raised to 38±2°C, and then the enzymolysis was kept at a constant temperature for 1-1.5 hours to complete the first enzymolysis. [0003] After the first enzymatic hydrolysis, add a pH regulator to adjust the pH value of the mixed solution, adjust the salinity of the mixed solution to 3.0-3.5 degrees, continue to heat up to 55±1℃, an...

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Problems solved by technology

[0004] In the process of enzymatic hydrolysis, a certain degree of heat preservation and stirring is required, so stirring equipment is required. After the preparation, the equipment needs to be cleaned to avoid the growth of internal bacteria. Conventional equipment on the market will be stirred during the cleaning process. During the process of taking out the shaft, due to the long length of the stirring shaft, it is necessary to lift the stirring shaft vertically upwards to remove the entire stirring shaft from the barrel. The stirring shaft can only be taken out by climbing a ladder. On the one hand, it is inconvenient to clean. On the other hand, climbing high is dangerous to workers. The mixing equipment on the market also has a stirring shaft in the form of a flip cover, but the flip cover requires an opening. The size of the mixer is matched with the length of the stirring shaft, so the stirring devices on the market are usually short in height, occupy a large area, but have a small capacity. Due to the long preparation time and low output, the heating method usually adopts Water bath heating method, but the heating tubes are all set at the bottom, and the heating process is from bottom to top, resulting in a large temperature difference between the upper and lower sides, which may easily lead to subsequent uneven temperature

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