Primary hepatocellular carcinoma molecular typing and survival risk gene group, diagnosis product and application

A hepatocellular carcinoma and molecular typing technology, which is applied in the field of primary hepatocellular carcinoma molecular typing, survival risk gene group and diagnostic products and applications, can solve the problems that clinicians and patients cannot guide treatment and predict prognosis, etc.

Active Publication Date: 2020-12-08
北京华奥铂瑞赛思医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no unified conclusion on the molecular classification of liver cancer, and the current research results can not play a good role in guiding treatment and predicting prognosis for clinicians and patients

Method used

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  • Primary hepatocellular carcinoma molecular typing and survival risk gene group, diagnosis product and application
  • Primary hepatocellular carcinoma molecular typing and survival risk gene group, diagnosis product and application
  • Primary hepatocellular carcinoma molecular typing and survival risk gene group, diagnosis product and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0115] Evaluation of subtypes of liver cancer and screening of survival risk-related gene groups

[0116] Methods: 370 cases in the TCGA database with complete Based on the expression level of primary liver cancer genes in clinical information, cell proliferation-related genes, immune-related genes, intercellular matrix-related genes, exosome-related genes, and FTH family genes that are closely related to the survival risk of liver cancer were screened out, and each group of genes Calculate and optimize the genes with a large contribution rate to typing and survival risk.

[0117] Results: A total of 62 genes and 6 reference genes related to liver cancer subtype and survival risk were screened, that is, 68 gene test combinations. See Table 1 for the list of genes.

[0118] The validity and stability of the 68 genes screened were verified in the Affymatrics gene chip expression profile data of 221 cases of primary liver cancer.

[0119] Table 1: Molecular classification of H...

Embodiment 2

[0124] Molecular typing and survival risk assessment of liver cancer by next-generation sequencing

[0125] Experimental method: A 68-gene test combination was used, of which 62 liver cancer subtypes and survival risk-related gene groups (proliferation-related genes BIRC5, BUB1, CCNB2, CDC20, CDC6, CDK1, E2F1, FOXM1, GINS1, KIF18B, KIF2C, MKI67 , MYBL2, PLK1, TOP2A, TPX2, TROAP, UBE2C, immune-related genes CD2, CD74, CD8A, PLA2G2D, SLAMF7, extracellular matrix-related genes ABCB11, ADH1B, ALDOB, APOF, AQP9, BHMT, CYP2A6, F9, FETUB, GLYAT, GYS2, HP, HPR, HRG, HSD17B6, RDH16, SERPINC1, SLC10A1, SLC27A5, TAT, TTR, exosome-related genes AEBP1, ANTXR1, APLNR, ASPN, CRISPLD2, DCN, EFEMP1, FBLN2, FMOD, GGT5, LAMC3 , LUM, MFAP4, MGP, MXRA5, MYH11, SVEP1, and FTH gene FTH1) were used for molecular typing and survival risk assessment, and six reference genes (including GAPDH, GUSB, MRPL19, PSMC4, SF3A1, and TFRC) were used as internal standards. All 62 genes except the 6 reference gene...

Embodiment 3

[0150] Molecular classification and survival risk assessment of liver cancer by quantitative PCR

[0151] Experimental method: A 24-gene test combination was used, of which 21 liver cancer subtypes and survival risk-related gene groups (proliferation-related genes: BIRC5, CDC20, MKI67, MYBL2, TOP2A, TPX2 and UBE2C; immune-related genes: CD2, CD74 and CD8A; intercellular matrix-related genes: GLYAT, GYS2, HRG, SERPINC1, SLC10A1, TAT; exosome-related genes: DCN, EFEMP1, LUM and MFAP4; FTH gene: FTH1) for molecular typing and survival risk assessment, 3 A reference gene (ACTB, GAPDH and RPLPO) was used as an internal standard. All 21 genes in Table 2 except the reference gene were used to calculate the survival risk index.

[0152] Table 2: Molecular classification of HCC and / or genogroups for survival risk

[0153]

[0154]

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Abstract

The invention discloses a primary hepatocellular carcinoma molecular typing and survival risk gene group, a diagnosis product and application. The gene group comprises 62 molecular typing and survivalrisk assessment related genes and 6 reference genes, wherein the 62 molecular typing and survival risk assessment related genes consist of 18 cell proliferation related genes, 5 immunity related genes, 21 extracellular matrix related genes, 17 exosome related genes and 1 FTH family gene. The diagnosis product comprises a reagent used for detecting an expression level of the genes in the gene group. The primary hepatocellular carcinoma molecular typing and survival risk gene group and the diagnosis product have higher accuracy in primary hepatocellular carcinoma molecular typing and survival risk assessment by screening the specific gene group and detecting the expression level of the genes in the gene group.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to molecular typing of primary hepatocellular carcinoma, survival risk gene group, diagnostic products and applications. Background technique [0002] Primary liver cancer is a highly lethal type of cancer with a high incidence in China, accounting for about half of the world's incidence. The incidence of primary liver cancer in China ranks fourth in the tumor spectrum, while the mortality rate ranks second. In 2015, there were 466,000 new liver cancer cases and 422,000 deaths in my country. At present, the precise treatment of liver cancer after surgery still faces great challenges at home and abroad. [0003] The general classification and histopathological classification of liver cancer have been used for a long time. For the same type and stage of liver cancer, no matter how optimized the clinical treatment is applied, the prognosis is still very different, and these differences ar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886G01N33/68G01N33/574
CPCC12Q1/6886G01N33/68G01N33/57438G01N33/57484C12Q2600/112C12Q2600/158C12Q2600/118
Inventor 周彤胡志元周伟庆马琳琳陆俊欢魏星
Owner 北京华奥铂瑞赛思医疗科技有限公司
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