Method for increasing yield of flavonoid compounds in young golden pomelo fruits

A technology of flavonoids and compounds, applied in botany equipment and methods, horticultural methods, horticulture, etc., to achieve the effect of increasing the content level, increasing the yield, and promoting the production amount

Inactive Publication Date: 2020-12-11
ZHONGKAI UNIV OF AGRI & ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For how to improve the output of flavonoids, current research mainly focuses on optimizing the extraction conditions and extraction techniques of flavonoids, such as the optimal extraction concentration of ethanol, the use of ultrasonic-assisted ethanol extraction, microwave-assisted ethanol extraction, etc., by increasing flavonoids The extraction rate of flavonoids is used to increase its yield, and from the perspective of promoting the production of flavonoids to improve its yield, there are few reports

Method used

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  • Method for increasing yield of flavonoid compounds in young golden pomelo fruits
  • Method for increasing yield of flavonoid compounds in young golden pomelo fruits
  • Method for increasing yield of flavonoid compounds in young golden pomelo fruits

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) Take the young pomelo fruit, and carry out collision damage treatment under the conditions of simulated collision, that is, make each young pomelo fruit fall freely from a height of about 1m from the landing point, repeat 10 times, and treat the young pomelo fruit. Exogenous damage stimulus;

[0030] (2) After carrying out the collision damage treatment for 5 days, take the pomelo young fruit which has been processed through the collision damage and wash them, cut them into small pieces, then dry and pulverize them, and pass through a 60-mesh sieve to obtain the pomelo young fruit powder;

[0031] (3) Add ethanol with a mass fraction of 82% to the young pomelo fruit powder according to the ratio of solid to liquid 1:20g / mL, ultrasonically extract at 60°C and 250W for 45min, filter, and collect the filtrate to obtain the total flavonoid extract, the total flavonoid extraction The liquid can be further obtained by freeze-drying and other drying methods to obtain dry p...

Embodiment 2

[0033] (1) Take the young pomelo fruit, and carry out collision damage treatment under the conditions of simulated collision, that is, make each young pomelo fruit fall freely from a height of about 1m from the landing point, repeat 10 times, and treat the young pomelo fruit. Exogenous damage stimulus;

[0034] (2) After carrying out the collision damage treatment for 6 days, the young pomelo fruit processed through the collision damage treatment was taken for cleaning, cut into small pieces, then dried, pulverized, and passed through a 60-mesh sieve to obtain young pomelo fruit powder;

[0035](3) Add ethanol with a mass fraction of 70% to the young pomelo fruit powder according to the ratio of material to liquid: 1:28g / mL, ultrasonically extract at 60°C and 250W for 71min, filter, and collect the filtrate to obtain the naringin extract, pomelo peel The glycoside extract can be further obtained by freeze-drying and other drying methods to obtain dry naringin powder.

Embodiment 3

[0037] (1) Take the young pomelo fruit, make each young pomelo fruit fall freely from a height of about 1m from the landing point, repeat 5 times;

[0038] (2) After carrying out the collision damage treatment for 4 days, take the pomelo young fruit which has been processed through the collision damage and wash them, cut into small pieces, then dry, pulverize, and pass through a 60-mesh sieve to obtain the pomelo young fruit powder;

[0039] (3) Add ethanol with a mass fraction of 75% to the young pomelo fruit powder according to the ratio of solid to liquid 1:30g / mL, ultrasonically extract at 60°C and 250W for 60min, filter, and collect the filtrate to obtain the total flavonoid extract, the total flavonoid extraction The liquid can be further obtained by freeze-drying and other drying methods to obtain dry powder of total flavonoids.

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Abstract

The invention discloses a method for increasing the yield of flavonoid compounds in young golden pomelo fruits. The method comprises the following steps of carrying out exogenous injury stimulation onthe young golden pomelo fruits, performing drying and pulverizing, and extracting the flavonoid compounds by using ethanol as an extraction solvent, wherein the flavonoid compounds can be general flavones or naringin. According to the method, the young golden pomelo fruits are subjected to pretreatment of exogenous damage stimulation, synthesis of the flavonoid compounds can be promoted, the content level of the flavonoid compounds in the young golden pomelo fruits is remarkably increased, and therefore the yield of the flavonoid compounds can be remarkably increased when the flavonoid compounds are extracted from the young golden pomelo fruits.

Description

technical field [0001] The invention relates to a method for increasing the yield of flavonoids in pomelo young fruit, in particular to a method for increasing the yield of flavonoids in pomelo young fruit by promoting the synthesis of total flavonoids and naringin in pomelo young fruit method. Background technique [0002] Flavonoids are an important natural active substance, a chemically diverse secondary metabolite, generally produced in plants, and can be divided into the following categories: flavonoids and dihydroflavonoids, chalcones And dihydrochalcones, flavonols and dihydroflavonols, isoflavones and dihydroisoflavones, etc. Flavonoids have a variety of biological activities and are widely used in medicine. For example, it has cardiovascular system activity, and many Chinese patent medicines that are effective in treating coronary heart disease contain flavonoids; it has: antibacterial and antiviral activities; antitumor activity; antioxidant free radical activity...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/06A01G17/00
CPCA01G7/06A01G17/005
Inventor 王琴刘袆帆梁楚容
Owner ZHONGKAI UNIV OF AGRI & ENG
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