Umbilical cord preservation fluid and umbilical cord preservation method

A preservation method and technology of preservation solution, which can be applied to the preservation, application, animal husbandry and other directions of human or animal bodies, and can solve the problems of not being able to preserve for a long time

Inactive Publication Date: 2020-12-11
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the above-mentioned prior art, the present invention provides an umbilical cord preservation solution and an umbil

Method used

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  • Umbilical cord preservation fluid and umbilical cord preservation method
  • Umbilical cord preservation fluid and umbilical cord preservation method
  • Umbilical cord preservation fluid and umbilical cord preservation method

Examples

Experimental program
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Embodiment 1

[0028] An umbilical cord preservation solution, calculated as 1L preservation solution, comprising:

[0029] Hyperbranched polyglycerol 18mmol, lacturonic acid 100mmol, potassium dihydrogen phosphate 25mmol, magnesium sulfate 5mmol, adenosine 5mmol, glutathione reduced form 3mmol, dexamethasone 8mg, allopurinol 1mmol, sucrose 60mmol, dextran-4050g, Verapamil 20mg, sodium hydroxide (50mmol / L) 5ml and potassium hydroxide (50mmol / L) 20ml.

[0030] The pH of the preservation solution in this embodiment is about 7.45, and the osmotic pressure is about 310mOSM / L.

Embodiment 2

[0032] An umbilical cord preservation solution, calculated as 1L preservation solution, comprising:

[0033] Hyperbranched polyglycerol 15mmol, lacturonic acid 101mmol, potassium dihydrogen phosphate 24mmol, magnesium sulfate 6mmol, adenosine 4mmol, glutathione reduced form 4mmol, dexamethasone 6mg, allopurinol 1.5mmol, sucrose 50mmol, dextran-4060g , verapamil 18mg, sodium hydroxide (50mmol / L) 6ml and potassium hydroxide (50mmol / L) 19ml.

[0034] The pH of the preservation solution in this embodiment is about 7.35, and the osmotic pressure is about 300mOSM / L.

Embodiment 3

[0036] An umbilical cord preservation solution, calculated as 1L preservation solution, comprising:

[0037] Hyperbranched polyglycerol 20mmol, lacturonic acid 99mmol, potassium dihydrogen phosphate 26mmol, magnesium sulfate 4mmol, adenosine 6mmol, glutathione reduced form 2mmol, dexamethasone 10mg, allopurinol 0.5mmol, sucrose 70mmol, dextran-4040g , verapamil 22mg, sodium hydroxide (50mmol / L) 4ml and potassium hydroxide (50mmol / L) 21ml.

[0038] The pH of the preservation solution in this embodiment is about 7.55, and the osmotic pressure is about 320mOSM / L.

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Abstract

The invention discloses an umbilical cord preservation fluid and an umbilical cord preservation method. The umbilical cord preservation fluid is prepared from 15-20 mmol of hyperbranched polyglycerol,99-101 mmol of lacturonic acid, 24-26 mmol of monopotassium phosphate, 4-6 mmol of magnesium sulfate, 4-6 mmol of adenosine, 2-4 mmol of glutathione reduction type, 6-10 mg of dexamethasone, 0.5-1.5mmol of allopurinol, 50-70 mmol of sucrose, 40-60 g of dextran, 18-22 mg of verapamil, 4-6 ml of sodium hydroxide and 19-21 ml of potassium hydroxide. When an umbilical cord is preserved , the cleanedumbilical cord is cut into small sections with the length of 3-5 cm, then immersed into a preservation liquid and preserved in an environment with the temperature of 3-5 DEG C. By adopting the preservation liquid, the problem that the umbilical cord cannot be preserved for a long time after being isolated can be effectively solved.

Description

technical field [0001] The invention belongs to the technical field of umbilical cord preservation, and in particular relates to an umbilical cord preservation solution and an umbilical cord preservation method. Background technique [0002] Mesenchymal stem cells (Mesenchymal stem cells, MSC) is an important member of the stem cell family, derived from the mesoderm in the early stage of development, and belongs to pluripotent stem cells. MSC was originally found in the bone marrow. The characteristics of differentiation potential, hematopoietic support and promotion of stem cell implantation, immune regulation and self-replication have attracted increasing attention. Previous studies have shown that MSCs have low immunogenicity, are able to evade immune detection, and have immunosuppressive properties and tissue repair / regenerative effects. [0003] Bone marrow-derived MSCs are the earliest and most in-depth study of mesenchymal stem cells, but the process of isolating bon...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/021A01N1/0226
Inventor 黄中力冯师健宋涂润
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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