A microchannel temperature control device and method for nucleic acid detection and amplification reaction

Abstract

A microchannel temperature control device and method for nucleic acid detection amplification reaction, relating to the microchannel temperature control device and method. The device includes a nucleic acid amplification reaction module, a heating / cooling module, a microchannel cooling module and a microcontroller module; the nucleic acid amplification reaction module and the microchannel cooling module are respectively installed on the upper and lower sides of the heating / cooling module; the nucleic acid amplification reaction The module includes a reaction plate, a reaction column and a reaction tube; the heating / cooling module is attached to the bottom of the reaction plate, and the heating / cooling and temperature control of the reaction module are realized through the microcontroller module; the microchannel cooling module includes a microchannel plate, a microchannel The cover plate, liquid pipe, heat dissipation fan, condenser, liquid receiver and water pump; the reaction column, thermoelectric cooling sheet and the outlet of the condenser are all equipped with thermocouples for collecting temperature signals. No outer insulation material is used, which improves the uniformity of temperature distribution, reduces costs and improves the convenience of production and operation. Increase the power of the thermoelectric cooling chip and increase the heating / cooling rate of the reaction module.

Description

technical field [0001] The invention relates to a microchannel temperature control device and method, in particular to a microchannel temperature control device and method for nucleic acid detection amplification reaction. Background technique [0002] In recent years, with the development of gene technology, nucleic acid detection has played an increasingly important role. Clinically, through nucleic acid detection, lesions can be detected in advance and treated as soon as possible, thereby improving the cure rate. At present, infectious diseases are prevalent and various viruses are raging, seriously affecting people's health. The new crown pneumonia epidemic has caused millions of people to be infected. COVID-19 has a long incubation period and there are asymptomatic infections, which pose great challenges to the prevention, control and treatment of the epidemic. At present, the most important means of epidemic prevention is large-scale nucleic acid testing to achieve ...

AI Technical Summary

Problems solved by technology

At present, the nucleic acid detection equipment on the market adopts the heat dissipation method of thermoelectric cooling sheets and fins. Due to the low heat dissipation efficiency of the fins, only low-power thermoelectric cooling sheets can be used, resulting in the temperature rise / fall rate of the samples in the reaction module. less than required

Especially in the cooling process, the hot end of the thermoelectric cooling chip generates a lot of heat when it is working, and the fins cannot export the heat in time, causing the heat to flow back to the cold end, further reducing the cooling effect, and the cooling rate is far from meeting the current detection requirements

On the other hand, due to heat exchange between the reaction columns and reaction tubes around the reaction module and the surrounding air, the temperature of each sample is inconsistent, resulting in inconsistent nucleic acid concentrations after sample amplification, which seriously affects the detection sensitivity and accuracy.

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