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Bacteria culture bottle containing beta-lactamase and preparation method thereof

A technology of bacterial culture and lactamase, applied in the medical field, can solve problems such as shortening the time of positive bacteria reporting, unreasonable application of antibiotics, and large differences in pediatric diagnosis and treatment, so as to improve the positive detection rate and shorten the time of positive bacteria reporting Effect

Pending Publication Date: 2020-12-11
TIANJIN CHILDRENS HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the level of pediatric diagnosis and treatment in my country is quite different, and there are different degrees of irrational use of antibiotics. The use of antibiotics before obtaining blood culture specimens will reduce the bacterial load in the specimens, inhibit the growth of bacteria, and ultimately affect the positive detection of blood cultures. Rate and TTP (time to positivity, TTP, time to report positive bacteria)
[0003] In order to effectively improve the positive rate of blood culture detection and shorten the time for positive bacteria reporting, culture bottles containing resin or activated carbon particles are often used to remove antibiotic residues in blood samples, but there are corresponding limitations
For example, the presence of activated carbon particles may be the main limiting factor in the application of Gram staining, direct mass spectrometry, molecular methods and direct antibiotic susceptibility testing; It is still not available in low- and middle-income countries; some studies have shown that the peak blood concentration of meropenem and a few cephalosporins may exceed the neutralization capacity of resin culture bottles

Method used

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  • Bacteria culture bottle containing beta-lactamase and preparation method thereof
  • Bacteria culture bottle containing beta-lactamase and preparation method thereof

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Embodiment Construction

[0012] Below in conjunction with the examples, the specific implementation of the present invention will be further described in detail. The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0013] The present embodiment provides that the present invention provides a bacterial culture bottle containing β-lactamase, which is added with a β-lactamase preparation capable of decomposing cephalosporins and penicillins antibiotics for treating blood Residual β-lactam antibiotics in the specimen.

[0014] This implementation also provides the preparation method of above-mentioned bacterial culture bottle, comprising:

[0015] According to the type and dose of β-lactam antibiotics taken by the child, according to the peak blood concentration of antibiotics in the corresponding drug instructions, and according to the ratio requirements of the β-lactamase preparation instructions, prepare the correspondi...

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Abstract

The invention relates to a bacteria culture bottle containing beta-lactamase and a preparation method thereof. The beta-lactamase capable of decomposing cephalosporins and penicillin antibiotics is added in the culture bottle to treat residual beta-lactamase antibiotics in blood specimens. The preparation method includes the following steps: preparing the beta-lactamase with corresponding dosage according to the types and dosages of the beta-lactamase antibiotics that child patients take, the plasma concentration of antibiotic peaks in corresponding package inserts and the proportioning requirements in the specification for beta-lactamase preparations; and simultaneously injecting the beta-lactamase and a clinical blood culture specimen into a clinically used bacteria culture bottle or a manually prepared bacteria culture bottle so as to obtain the bacteria culture bottle containing the beta-lactamase. Thus, the positive detection rate of blood culture can be increased, and time to positivity can be shortened.

Description

technical field [0001] The invention belongs to the field of medical technology, in particular to a bacterial culture bottle containing β-lactamase and a preparation method thereof. Background technique [0002] Sepsis is one of the leading causes of death in adults and children worldwide, with the highest morbidity and mortality among newborns and children, and has not declined significantly over the past few decades. The incidence of sepsis in children ranges from 0.56 to 2.1 per 1,000 children per year. Guidelines for the management of sepsis almost all emphasize the importance of collecting blood for blood cultures before administering antibiotics. However, more than 40% of hospitalized patients received antibiotics before blood collection and culture, and about 20% of patients had already used oral antibiotics on admission. Blood culture has important clinical significance for the diagnosis, treatment and prognosis of infectious diseases. However, the level of pediat...

Claims

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Application Information

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IPC IPC(8): C12M1/24C12M1/00
CPCC12M23/08
Inventor 刘洋陈楠张莹李娜
Owner TIANJIN CHILDRENS HOSPITAL
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