Composition for autologous lymphocyte culture, culture solution and application of composition

A technology of lymphocytes and compositions, applied in the field of lymphocyte culture, which can solve the problems of restricting wide application and difficult quality control

Active Publication Date: 2020-12-15
北京奥康华医学检验所有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing in vitro induced and expanded autologous lymphocyte preparation technology cannot be industrialized and the quality is difficult to control, which limits its wide clinical application

Method used

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  • Composition for autologous lymphocyte culture, culture solution and application of composition
  • Composition for autologous lymphocyte culture, culture solution and application of composition
  • Composition for autologous lymphocyte culture, culture solution and application of composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1. Using the composition and culture medium for culturing lymphocytes of the present invention to prepare autologous lymphocytes

[0042] 1. Preparation of autologous lymphocyte culture medium

[0043] 1.1 Preparation of human autologous plasma

[0044] Place the human autologous plasma in a 56°C water bath for 30 minutes, place it in a refrigerator at 2-8°C for at least 10 minutes, centrifuge at a low temperature of 2000rpm for 10 minutes, remove the precipitate to obtain human autologous plasma, mark the production batch number, and store it at 2-8°C for future use.

[0045] 1.2 Preparation of autologous lymphocyte culture medium

[0046] 1.2.1 Preparation of autologous lymphocyte culture fluid of the present invention

[0047] The autologous lymphocyte culture fluid of the present invention is prepared by using the composition for culturing lymphocytes of the present invention. The composition for culturing lymphocytes of the present invention consists of ...

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PUM

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Abstract

The invention discloses a composition for autologous lymphocyte culture, a culture solution and application of the composition. The active ingredients of the composition for autologous lymphocyte culture are composed of plasma, an anti-human CD3 monoclonal antibody, IL-2, IL-7, IL-15 and PHA-P. Compared with a composition for lymphocyte culture as a control, the content of a T lymphocyte subset obtained by using a culture medium containing the composition for induced culture of peripheral blood mononuclear cells is significantly increased; the number of lymphocytes is also significantly increased after 14 days of culture, and the number is increased by 1.15 times; and the killing activity of the lymphocytes on K562 cells is also significantly enhanced, and the killing activity is enhancedby 1.6 times. The composition for autologous lymphocyte culture can be used for specific induced amplification of mononuclear cells to produce autologous lymphocytes with high purity and strong biological activity.

Description

technical field [0001] The invention relates to the technical field of lymphocyte culture, in particular to a culture solution for autologous lymphocyte culture. Background technique [0002] As a heterogeneous cell population, lymphocytes include many subpopulations with different immune functions. It is an important cell component of the body's immune response function, the main executor of almost all immune functions of the lymphatic system, and the first-line defense system for the body to resist external infections and monitor cell variation in the body. As a kind of cell line with immune recognition function, lymphocytes can be divided into T lymphocytes, B lymphocytes and natural killer (NK) cells according to their migration, surface protein molecules and functions. Adoptive immunotherapy of lymphocytes refers to inducing and expanding lymphocytes with anti-tumor activity in vitro, adopting them in patients, and activating the body's cellular and humoral immune resp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0783C12N5/0781A61K35/17A61P35/00
CPCC12N5/0636C12N5/0635C12N5/0646A61K35/17A61P35/00C12N2501/2302C12N2501/2307C12N2501/2315C12N2501/59C12N2501/515C12N2500/84C12N2501/24C12N2506/11
Inventor 卢戌刘静维王跃刘雪松李君阳
Owner 北京奥康华医学检验所有限公司
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