High-resolution spectral image rapid acquisition device and method

Abstract

The invention discloses a high-resolution spectral image rapid acquisition device and method. The device comprises an illumination light source, an objective lens, a spectroscope, a single-shot spectral image acquisition assembly, and a reference image acquisition assembly. The illumination light source is used for projecting illumination light onto the sample to be detected so that the sample tobe detected is amplified by the objective lens, the amplified light is divided into two parts of light by the spectroscope; one part of light enters the single-shot spectral image acquisition assemblyso as to acquire a low-resolution spectral cube of the sample to be detected, and the other part of the light enters the reference image acquisition assembly to acquire a high-resolution spectral cube. According to the invention, the high-resolution spectral image can be rapidly obtained, so that the process of applying the spectral image to medical diagnosis is accelerated.

Description

technical field [0001] The invention relates to the field of image technology, in particular to a device and method for quickly acquiring high-resolution spectral images. Background technique [0002] Hyperspectral images have two spatial dimensions and one spectral dimension, which can provide information about tissue physiology, morphology, and composition, and thus are widely used in disease diagnosis and image-guided surgery. Cells in a disease state have different structures or metabolic rates from those in a normal state, and thus have different fluorescence emission spectra. Therefore, fluorescence hyperspectral images have the ability to diagnose diseases in the early stages of diseases. [0003] At present, in the field of pathological imaging, spectral images and fluorescence spectral images are mostly obtained by point scanning spectroscopic method (pushbroom) and switching filter method (staring), but these two methods will cause huge time expenditure, and switch...

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Problems solved by technology

[0003] At present, in the field of pathological imaging, spectral images and fluorescence spectral images are mostly obtained by means of point-scanning spectroscopy (pushbroom) and switching filter (staring), but these two methods will cause huge time expenditures, and switching filters The light sheet needs to constantly replace the filter, and it will inevitably cause mechanical vibration during the image acquisition process, which will reduce the quality of the acquired image; therefore, these defects in the existing spectral image acquisition methods seriously hinder the use of spectral images for disease diagnosis. in the development of clinical medicine

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