Application of lactobacillus johnsonii in preventing and/or treating inflammatory bowel diseases

A technology for Lactobacillus johnsonii and inflammatory bowel disease, applied in the field of oral microecological preparations, can solve problems such as insignificant curative effect, and achieve the effect of improving symptoms

Active Publication Date: 2020-12-25
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A large number of studies have shown that a variety of probiotics such as lactic acid bacteria and bifidobacteria can alleviate inflammatory bowel disease, but there are still some disadvantages, such as the curative effect is not obvious

Method used

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  • Application of lactobacillus johnsonii in preventing and/or treating inflammatory bowel diseases
  • Application of lactobacillus johnsonii in preventing and/or treating inflammatory bowel diseases
  • Application of lactobacillus johnsonii in preventing and/or treating inflammatory bowel diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1. Isolation, identification and cultivation of Lactobacillus johnsonii

[0061] 1.1. Isolation of Lactobacillus johnsonii

[0062] Place mouse colonic digesta in a 1.5ml centrifuge tube, add 500μl PBS, and vortex for 1 minute to make it completely suspended. The obtained suspension was diluted with 10-fold serial gradient, spread on MRS (Man, Rogosa and Sharpe) agar plate, and incubated overnight at 37°C.

[0063] 1.2. Identification of Lactobacillus johnsonii

[0064] Pick the single clone of Lactobacillus johnsonii in the MRS liquid medium, amplify the full length of 16S rDNA after static culture, and the primer sequences are respectively:

[0065] 27F: 5'-AGAGTTTGATCCTGGCTCAG-3';

[0066] 1492R: 5'-CGGYTACCTTGTTACGACTT-3'.

[0067] The PCR reaction system is shown in Table 1:

[0068] Table 1. PCR reaction system

[0069]

[0070] The PCR reaction conditions are as follows:

[0071]

[0072] The obtained PCR products were detected by 1% agarose ...

Embodiment 2

[0076] Example 2. Prevention and treatment of Lactobacillus johnsonii on mice with inflammatory bowel disease

[0077] 2.1. Cultivation of Citrobacter

[0078] Citrobacter (DBS100) was activated twice and then inoculated into an Erlenmeyer flask containing 50mL of LB (Luria-Bertani) medium, sealed to form a micro-aerobic environment, cultured in a constant temperature shaker at 37°C, 220rpm for 12 hours, and set aside.

[0079] 2.2. Establishment, grouping and administration of inflammatory bowel disease mouse model

[0080] After a one-week adaptation period, the mice were randomly divided into 4 groups according to body weight, 12 in each group, including:

[0081] Control group: mice in this group were fed with PBS every 2 days;

[0082] Illness group (i.e. Citrobacter gavage group): mice in this group were fed with PBS once every 2 days, and on the 8th day were fed with 2×10 9 CFU of Citrobacter, and then continued to feed for a week, wherein PBS was fed once every 2 da...

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Abstract

The invention belongs to the field of biological medicines, and discloses an application of lactobacillus johnsonii in preparing a medicine for preventing and/or treating inflammatory bowel diseases,and an oral micro-ecological preparation for preventing and/or treating inflammatory bowel diseases. The micro-ecological preparation comprises lactobacillus johnsonii, such as a UMNLJ22 strain. The lactobacillus johnsonii can significantly improve spleen proliferation, colon shortening, colonic mucosa proliferation and colitis reaction in a Citrobacter rodentium-induced inflammatory bowel diseasemouse model, and it is found that the lactobacillus johnsonii can significantly relieve inflammatory bowel diseases of mice, so that the lactobacillus johnsonii is used for development and preparation of drugs for preventing and/or treating inflammatory bowel diseases.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to the application of Lactobacillus johnsonii in the preparation of medicines for preventing and / or treating inflammatory bowel disease and oral probiotics containing the Lactobacillus johnsonii. Background technique [0002] Inflammatory bowel disease (Inflammatory bowel disease, IBD) is a chronic, easily relapsing inflammatory bowel disease. The clinical symptoms are mainly abdominal pain, abdominal distension, diarrhea and blood in the stool. In the past few decades, the incidence of inflammatory bowel disease in the world has increased year by year, which makes inflammatory bowel disease an important public health problem worldwide. [0003] Although the research on inflammatory bowel disease has become more complete and systematic in recent years, the etiology and pathogenesis of inflammatory bowel disease are still not very clear. Recent studies have shown that the factors affecting ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/747A61K31/60A61P1/00A61P29/00A61P1/04C12N1/20C12R1/225
CPCA61K35/747A61P1/00A61P29/00A61P1/04C12N1/20A61K31/60C12R2001/225C12N1/205A61K2300/00Y02A50/30
Inventor 武振龙张云常杨鹰
Owner CHINA AGRI UNIV
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