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A rice osabcc9 gene and its application in transporting cadmium

A rice and genetic technology, applied in the fields of application, genetic engineering, plant genetic improvement, etc.

Active Publication Date: 2022-08-09
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, no new rice ABC transporter family genes have been found to be cadmium-tolerant in the existing reports

Method used

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  • A rice osabcc9 gene and its application in transporting cadmium
  • A rice osabcc9 gene and its application in transporting cadmium
  • A rice osabcc9 gene and its application in transporting cadmium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0105] 1. Plant material and growing conditions

[0106] The materials used in this example include wild-type Nipponbare rice and two OsABCC9 mutant plants.

[0107] The rice seeds were soaked in tap water and placed in a 28°C incubator in the dark for 2 days after germination. 2 In a 4L plastic container with deionized water, the culture was continued for 2 days at 28 °C. After the roots grow to 2-3 cm, they are used for subsequent related experiments.

[0108] 2. Obtaining the full sequence of OsABCC9 gene and constructing the phylogenetic tree

[0109] To obtain the CDS sequence of OsABCC9, total RNA was extracted from rice roots using Trizol kit (Lifetechnologies) according to the product's instructions. Take 1 μg of total RNA and use the Hiscript II Q RT SuperMix Kit (Vazyme) synthesis kit to synthesize the first piece of cDNA. The obtained cDNA was used as a template to obtain the complete sequence of OsABCC9 in the following different experiments.

[0110] Phylogen...

Embodiment 2

[0122] 1. Using the method of PEG-mediated transformation, the plasmid GFP-OsABCC9 or GFP empty vector obtained in Example 1 was co-transformed into rice protoplast cells with the tonoplast marker plasmid AtTPK1, and after incubation at room temperature, confocal laser light was used. Scanning microscope (TCS SP8; Leica) to take pictures, the results are as follows Figure 5 shown.

[0123] Depend on Figure 5 As shown in A-D, the GFP empty vector (green fluorescence) is localized in the cytoplasm and nucleus, while the tonoplast marker AtTPK1 (red fluorescence) is only localized on the tonoplast. However, by Figure 5 As shown in E-H in the middle, the co-localization of GFP-OsABCC9 and AtTPK1 showed that green fluorescence and red fluorescence could be fused, indicating that OsABCC9 was localized on the tonoplast membrane.

[0124] 2. Phenotypic analysis and Cd content analysis of OsABCC9 mutant plants

[0125] In order to study the Cd tolerance of OsABCC9 mutant plants,...

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Abstract

The invention provides a rice OSABCC9 gene and its application, which belongs to the field of plant gene engineering technology, which is shown in the nucleotide sequence of the rice OSABCC9 gene as SEQ ID NO.1.The rice OSABCC9 gene provided by the present invention has the effect of transporting cadmium, and the protein encoded of rice OSABCC9 genes plays an important role in the process of decrypting cadmium.The expression of the OSABCC9 gene in the present invention is highly induced by the CD in the rice root; the analysis of sub -cell positioning shows that OSABCC9 is positioned on the liquid blister film; plant experiment analysis shows that the OSABCC9 gene has the function of transferring CD.The OSABCC9 gene of the present invention can be used as a cadmium transport protein, which plays an important role in the process of cadmium decryption poison of rice.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering, in particular to a rice OsABCC9 gene and its application in transporting cadmium. Background technique [0002] Cadmium (Cd) is one of the metal elements that are highly toxic to animals and plants. Excess cadmium in soil can be absorbed by plant roots, thereby inhibiting plant growth and reducing crop yield. Moreover, the cadmium absorbed by the roots can be transferred from the roots to the aerial parts, and then accumulated in fruits and grains, and entered into the human body through dietary intake. Long-term intake of toxic cadmium can cause renal insufficiency, fractures, cancer, etc. various diseases. Therefore, elucidating the transport process and accumulation mechanism of Cd in plants is helpful for the production and development of low-Cd crops, thereby reducing or reducing the uptake of Cd in humans. [0003] As a non-essential element, Cd mainly enters the root c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/82A01H5/00A01H6/46
CPCC07K14/415C12N15/8271
Inventor 夏继星付珊黄晶晶杨广哲
Owner GUANGXI UNIV