Bacillus cereus B-28 and application thereof

A technology of Bacillus cereus and B-28, which is applied in the field of soil pollution biological treatment, can solve the problems of complex colony, many influencing factors, and inability to strictly control experimental conditions, etc., to reduce Cd absorption, promote growth, and reduce Cd biological Effectiveness

Active Publication Date: 2020-12-18
JINAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, the Chinese invention patent with a publication date of September 7, 2016 and a publication number of CN105925507A discloses Bacillus cereus B19 which has functions of inactivating heavy metals and promoting plant growth; When preventing plants from absorbing Cd, the existing technology is directly carried out through soil culture, but there are many influencing factors in the soil, and the bacterial colonies are complex. In addition to Bacillus cereus, there are other bacterial species that may play a role, and the experiment cannot be strictly controlled. condition

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  • Bacillus cereus B-28 and application thereof
  • Bacillus cereus B-28 and application thereof
  • Bacillus cereus B-28 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The adsorption effect of embodiment 1 bacillus cereus B-28 to water-soluble state Cd

[0037] 1. Experimental method

[0038]Inoculate Bacillus cereus B-28 in LB medium and cultivate overnight, centrifuge at 6000rpm for 10min, discard the supernatant, then wash the bacteria twice with sterile water, and finally resuspend in sterile water to obtain a spare bacterial solution. Then inoculate 0.5 mL of bacterial liquid into 100 mL of LB medium containing 1 mg / L Cd (added in the form of Cd(NO3) 2 ), and inoculate into LB medium without Cd as a control, and each treatment has three parallels. Place in a shaker at 180rpm and culture at 30°C. On the 1st, 3rd, 5th, and 7th days of bacterial strain culture, samples were taken once to measure the OD of the bacterial liquid 600 Value and pH value, then the bacterial solution was centrifuged at 6000rpm for 10min to obtain bacterial pellet. The harvested cells were washed twice with sterile water, and then 20 mL of 10 mM EDTA sol...

Embodiment 2

[0042] Example 2 Effect of inoculation of Bacillus cereus B-28 on corn absorption of Cd

[0043] 1. Experimental method

[0044] In this study, through sand culture experiments, five different Cd concentrations were set to explore the effects of Bacillus cereus B-28 on the growth and cadmium absorption of maize. A total of 10 treatments were used in the experiment, which were: 0.03J (the concentration of Cd added to the potted plant was 0.03mg / kg quartz sand, the same as the following; inoculation), 0.06J (the concentration of Cd was 0.06mg / kg, inoculation), 0.09J (Cd Concentration 0.09mmg / kg, inoculation), 0.12J (Cd concentration 0.12mg / kg, inoculation), 0.15J (Cd concentration 0.15mg / kg, inoculation) and 5 non-inoculation controls of each Cd concentration: 0.03 CK, 0.06CK, 0.09CK, 0.12CK, and 0.15CK. There were 4 parallels for each treatment, a total of 40 pots, and each pot contained 4kg of quartz sand.

[0045] Corn seeds were treated with 10% H before use 2 o 2 Disin...

Embodiment 3

[0049] The direct impact of embodiment 3 inoculating Bacillus cereus B-28 on the growth of corn

[0050] 1. Experimental method

[0051] The corn was harvested after 100 days of growth. When harvesting, first measure the height of the corn plant, shake the corn root to wash, thereby separating the rhizosphere sand, wash it with deionized water and dissolve it in 0.5mM CaCl 2 Soak in water for 0.5h to remove the Cd attached to the corn root surface, and wipe the surface with absorbent paper to remove the water attached to the root surface. The corn tissue was divided into roots, stems, leaves, corn kernels and corn cobs, and the fresh weights were weighed separately, and the fresh samples of each reserved part were immediately quenched with liquid nitrogen and stored in a -80°C refrigerator (Haier DW-86L626). For the determination of enzyme activity and chlorophyll, the remaining tissue samples were put into envelopes and placed in a constant temperature oven at 105°C for 0.5...

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Abstract

The invention discloses a strain of bacillus cereus B-28 and application thereof. A strain of bacillus cereus B-28 is obtained through separation and screening in the invention; the strain is preserved in the Guangdong microbial culture collection centre on July 13th, 2020; and the preservation number of the strain is GDMCC NO:61089. The strain has relatively strong heavy metal Cd passivation capability, has the capability of reducing heavy metal Cd absorption by plants, meanwhile, can repair heavy metal Cd contaminated soil, and can significantly promote the increase of the chlorophyll content of corn leaves and the enhancement of the activity of CAT and POD enzymes at roots, so as to promote the increase of biomass of each tissue of corn; and therefore, the strain has very wide application prospect in repair of heavy metal Cd contaminated soil, promotion of plant growth and prevention and control of heavy metal Cd absorption by plants.

Description

technical field [0001] The invention belongs to the technical field of soil pollution biological treatment. More specifically, it relates to a strain of Bacillus cereus B-28 and its application. Background technique [0002] Cadmium is one of the main culprits of heavy metal pollution in farmland soil in my country. Due to its high biological toxicity and high mobility, it has already posed a serious threat to the safety of agricultural products. How to reduce the risk of soil cadmium pollution and reduce the absorption of cadmium by crops has become an urgent task. As a promising direction, microbial passivation technology means that microorganisms can transform through valence (such as Cr(VI) into Cr(III), SeO 4 2- Conversion to Se), biosorption (such as microbial extracellular polymer adsorption), chemical precipitation and volatilization (such as conversion of methylmercury to mercury vapor), etc. to convert the chemical form of heavy metals or reduce the toxicity of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10A01G22/20A01G7/06C12R1/085
CPCC12N1/20B09C1/10A01G22/20A01G7/06A01N63/22C09K17/14C09K2101/00C12R2001/085C12N1/205
Inventor 李取生阮翔李万里范瀚允侯思颖徐子杰
Owner JINAN UNIVERSITY
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