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Application of SOX11 gene in detecting and/or regulating myocardial cell excessive apoptosis

A technology of SOX11, cardiomyocytes, applied in the field of molecular biology

Active Publication Date: 2021-01-01
国家卫生健康委科学技术研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the relationship between the expression of SOX11 and the excessive apoptosis of cardiomyocytes

Method used

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  • Application of SOX11 gene in detecting and/or regulating myocardial cell excessive apoptosis
  • Application of SOX11 gene in detecting and/or regulating myocardial cell excessive apoptosis
  • Application of SOX11 gene in detecting and/or regulating myocardial cell excessive apoptosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0090] Establish a hyperglycemia pregnant rat model (or called: high glucose group) as follows:

[0091] Female and male SD (Sprague-Dawley) rats (10-11 weeks old) were given free access to food and Rearing was carried out in metabolic cages by means of water.

[0092] Streptozotocin (STZ) was used to induce diabetes in rats to obtain rats in the high glucose group. The specific operation is as follows:

[0093] Female SD rats were housed together with male SD rats. If a mating plug is observed the next morning, the day of seeing the mating plug is considered to be day 0.5 of pregnancy. After seeing the mating plug, they were fasted for 12 hours, and STZ was intraperitoneally injected into the pregnant mice according to the standard of 50 mg / kg. After 72 hours, blood was taken from the tip of the tail, and the blood sugar level of the mother rats was measured with a blood glucose meter. The pregnant rats whose blood sugar level reached or exceeded 16.7mM were the hyperglyc...

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PUM

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Abstract

The invention belongs to the field of molecular biology, provides a preparation for detecting myocardial cell excessive apoptosis and a method for detecting myocardial cell excessive apoptosis, and further provides application of SOX11 gene overexpression plasmid in preparing a preparation for reducing the myocardial cell apoptosis rate in a high-glucose environment, application of siRNA interference plasmid of the SOX11 gene in preparing a preparation for increasing the myocardial cell apoptosis rate in the high-glucose environment, and a method for regulating the myocardial cell apoptosis rate. By means of the relation between the SOX11 gene and myocardial cell excessive apoptosis, especially the relation between the SOX11 gene and myocardial cell excessive apoptosis in the high-glucoseenvironment, the provided preparation for detecting myocardial cell excessive apoptosis can efficiently and accurately detect the myocardial cell apoptosis condition in the high-glucose environment, and therefore whether excessive apoptosis happens or not can be accurately judged for development of heart tissue during development of in-vitro cultured myocardial cells or maternal tubes.

Description

technical field [0001] The invention belongs to the field of molecular biology, and particularly relates to the application of SOX11 gene in detecting and / or regulating excessive apoptosis of cardiomyocytes, especially the application of excessive apoptosis of cardiomyocytes caused by high glucose environment. Background technique [0002] Congenital heart disease (CHD) refers to a group of congenital malformations with abnormal shape, structure and function caused by the heart and blood vessel development disorders during fetal period. Congenital heart disease is one of the leading causes of infant death. In Asia, CHD affects 9.3 per 1000 live births. [0003] The exact cause of CHD remains unknown. Over the years, studies have confirmed that CHD is a polygenic genetic disease, which is a typical complex trait disease caused by cardiovascular genetic factors and environmental factors in the embryonic period, and its heritability is 55% to 65%. Therefore, studying the pat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12Q1/6851G01N33/68A61K48/00A61P9/00
CPCC12Q1/6883C12Q1/6851G01N33/6893A61K48/005A61P9/00C12Q2600/158G01N2800/32G01N2333/47C12Q2531/113C12Q2521/107
Inventor 苏冬梅马旭高倩倩关丽娜李乾石翠格
Owner 国家卫生健康委科学技术研究所
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