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Application of MST1 gene in detecting and/or regulating excessive apoptosis of myocardial cells

A cardiomyocyte and gene technology, applied in the field of molecular biology, can solve the problem that there is no report of cardiomyocyte apoptosis

Pending Publication Date: 2021-01-29
国家卫生健康委科学技术研究所
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although Survivin has been well studied in tumors, there is no report on its relationship with cardiomyocyte apoptosis in a high glucose environment

Method used

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  • Application of MST1 gene in detecting and/or regulating excessive apoptosis of myocardial cells
  • Application of MST1 gene in detecting and/or regulating excessive apoptosis of myocardial cells
  • Application of MST1 gene in detecting and/or regulating excessive apoptosis of myocardial cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0104] Establish a hyperglycemia pregnant rat model (or called: high glucose group) as follows:

[0105] Female and male SD (Sprague-Dawley) rats (10-11 weeks old) were given free access to food and Rearing was carried out in metabolic cages by means of water.

[0106] Streptozotocin (STZ) was used to induce diabetes in rats to obtain rats in the high glucose group. The specific operation is as follows:

[0107] Female SD rats were housed together with male SD rats. If a mating plug is observed the next morning, the day of seeing the mating plug is considered to be day 0.5 of pregnancy. After seeing the mating plug, they were fasted for 12 hours, and STZ was intraperitoneally injected into the pregnant mice according to the standard of 50 mg / kg. After 72 hours, blood was taken from the tip of the tail, and the blood sugar level of the mother rats was measured with a blood glucose meter. The pregnant rats whose blood sugar level reached or exceeded 16.7mM were the hyperglyc...

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Abstract

The invention belongs to the field of molecular biology, and provides a preparation for detecting myocardial cell excessive apoptosis and a method for detecting myocardial cell excessive apoptosis. The invention also provides an application of the MST1 gene expression plasmid in preparation of a preparation for improving the myocardial cell apoptosis rate in a high-glucose environment, an application of the siRNA interference plasmid of the MST1 gene in preparation of a preparation for reducing the myocardial cell apoptosis rate in the high-glucose environment, and a method for regulating andcontrolling the myocardial cell apoptosis rate. By utilizing the relationship between the MST1 gene and myocardial cell excessive apoptosis, especially myocardial cell excessive apoptosis in a high-glucose environment, the preparation for detecting myocardial cell excessive apoptosis provided by the invention can be used for efficiently and accurately detecting the apoptosis condition of myocardial cells in the high-glucose environment; and therefore, whether excessive apoptosis occurs to cardiac muscle cells cultured in vitro or development of heart tissue during fetal development in a motherbody or not is accurately judged.

Description

technical field [0001] The invention belongs to the field of molecular biology, and particularly relates to the application of MST1 gene in detecting and / or regulating excessive apoptosis of cardiomyocytes, especially the application of excessive apoptosis of cardiomyocytes caused by high glucose environment. Background technique [0002] Congenital heart disease (CHD) refers to a group of congenital malformations with abnormal shape, structure and function caused by the heart and blood vessel development disorders during fetal period. Congenital heart disease is one of the leading causes of infant death. In Asia, CHD affects 9.3 per 1000 live births. [0003] The exact cause of CHD remains unknown. Over the years, studies have confirmed that CHD is a polygenic genetic disease, which is a typical complex trait disease caused by cardiovascular genetic factors and environmental factors in the embryonic period, and its heritability is 55% to 65%. Therefore, studying the path...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883G01N33/573A61K48/00A61K31/713A61P9/00
CPCC12Q1/6883G01N33/573A61K31/713A61P9/00C12Q2600/158G01N2800/32
Inventor 苏冬梅马旭关丽娜石翠格李乾
Owner 国家卫生健康委科学技术研究所
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