Micro fluidic chip for nucleic acid detection

Abstract

The invention discloses a micro fluidic chip for nucleic acid detection use. The micro fluidic chip for the nucleic acid detection use comprises an air passage layer, an interface layer, and a flow passage layer; the air passage layer consists of buttons one to four as well as air passages thereof, a reaction liquid storage tank, an eluent storage tank, a cleaning liquid storage tank, and a sampleliquid storage tank; the interface layer is an elastic film; the flow passage layer consists of a waste liquid tank, a nucleic acid purification and concentration chamber, a reaction chamber, and a plurality of flow passages; the reaction liquid storage tank, the eluent storage tank, the cleaning liquid storage tank and the sample liquid storage tank are respectively connected with flow passagesone to four on the flow passage layer via through holes in the film; four micro pumps are separately arranged on the flow passages one to four; the flow passages one to three are connected with an inlet of the nucleic acid purification and concentration chamber; the nucleic acid purification and concentration chamber is separately connected with the waste liquid tank and the reaction chamber through flow passages five to six; the reaction liquid storage tank is connected with the reaction chamber through the flow passage four; the buttons one to four are respectively connected with the micro pumps through the air passages; and liquid is pumped out of the liquid storage tank through the corresponding micro pumps when being pressed, thereby realizing quantitative flow of liquid.

Description

technical field [0001] The invention relates to the fields of microfluidic chip technology and molecular diagnosis, in particular to a microfluidic chip for nucleic acid detection. Background technique [0002] The concept of micro total analysis system was first proposed in the 1990s. Since then, on the basis of microelectronics, micromechanics, bioengineering and nanotechnology, microfluidic technology has developed rapidly and has become one of the most cutting-edge scientific and technological fields in the world. At present, its core technology is a microfluidic chip based on microfluidic technology, also known as a lab on chip (Lab on chip). Microfluidic chips are widely used in the biomedical field because of their advantages such as low consumption, low cost, high throughput, and automatic operation. One of the important applications is molecular diagnostic technology based on microfluidic chips. [0003] At the beginning of 2020, a new type of coronavirus spread ar...

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Problems solved by technology

Although the above products change nucleic acid detection from manual to aut

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