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A fully human antagonistic antibody targeting connective tissue growth factor and its application

A technology of growth factor and connective tissue, applied in the field of genetic engineering and protein engineering

Active Publication Date: 2022-02-11
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Human connective tissue growth factor is a protein that has been found to be closely related to the onset and progression of rheumatoid arthritis, but so far no inhibitors targeting human connective tissue growth factor have been screened from human combinatorial antibody libraries using phage display and other technologies. Antibody literature and products

Method used

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  • A fully human antagonistic antibody targeting connective tissue growth factor and its application
  • A fully human antagonistic antibody targeting connective tissue growth factor and its application
  • A fully human antagonistic antibody targeting connective tissue growth factor and its application

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Embodiment Construction

[0025] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings.

[0026] The present invention provides a fully human antagonistic antibody targeting connective tissue growth factor, which is a single-chain antibody, which has the specificity of targeting human connective tissue growth factor, and its amino acid sequence is SEQ ID NO: 1, SEQ ID NO: 2, Shown in SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9 or SEQ ID NO: 10.

[0027] The fully human antagonistic antibody targeting connective tissue growth factor of the present invention can be fused with other antibody drugs for treating rheumatoid arthritis, and the formed antibody can play the role of targeting human connective tissue growth factor on the one hand, and on the other On the one hand, it can play a role in the trea...

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Abstract

The invention relates to the technical fields of genetic engineering and protein engineering, in particular to a fully human antagonistic antibody targeting connective tissue growth factor and its application. Wherein the fully human antagonistic antibody targeting connective tissue growth factor is a single-chain antibody, and its amino acid sequence is SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5 , SEQIDNO:6, SEQIDNO:7, SEQIDNO:8, SEQIDNO:9 or SEQIDNO:10. The present invention screens out a fully human anti-CTGF monoclonal antibody through the ScFv phage antibody library of patients with rheumatoid arthritis, and determines its nucleotide sequence and amino acid sequence respectively. The fully human antagonistic antibody obtained by this method has the ability to target human Specificity of connective tissue growth factor.

Description

technical field [0001] The invention relates to the technical fields of genetic engineering and protein engineering, in particular to a fully human antagonistic antibody targeting connective tissue growth factor and its application. Background technique [0002] Rheumatoid arthritis is an autoimmune disease with complex etiology and recurrent attacks. Its pathological features are chronic inflammation of joint synovium, cartilage absorption, bone destruction and fibrosis, which has caused great inconvenience and pain to the majority of patients. . Because its etiology is not yet fully clarified, and its pathogenesis involves many aspects such as genetics, environment, immunity, etc., it is difficult for current drugs to cure patients, and only symptomatic treatment can be achieved, that is, to reduce or eliminate joint redness, swelling and pain caused by joint inflammation. Control the development of the disease, reduce joint damage, and ensure the function of the affected...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/22C12N15/13A61K39/395A61P19/02A61P29/00
CPCC07K16/22C07K16/005A61P19/02A61P29/00C07K2317/622C07K2317/76A61K2039/505
Inventor 王建光吴淦金胜威杨新宇
Owner WENZHOU MEDICAL UNIV