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Artificially synthesized herbicide-resistant protein

A protein and protein technology, applied in the biological field, can solve the problems of cotton that have not been reported yet

Active Publication Date: 2021-01-26
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, the acreage of commercial crops such as corn, soybean, cotton, and rapeseed that are resistant to glyphosate herbicides has increased year by year, and the economic benefits have been significant. However, there is no commercial herbicide-resistant cotton that can be used in China. to report

Method used

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  • Artificially synthesized herbicide-resistant protein

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1, EPSPS fusion gene TS-EPSPS

[0040] All the following gene manipulation methods were carried out with reference to the description in "Molecular Cloning" (Sambrook J et al., 1989); the restriction endonucleases and other tool enzymes used were all purchased from NEB Company.

[0041] 1. Acquisition of EPSPS fusion gene TS-EPSPS and fusion gene ACP1-EPSPS

[0042] 1. EPSPS codon optimization transformation and chemical synthesis

[0043] The codon optimization strategies for the herbicide-resistant protein coding gene (EPSPS gene) sequence of wild-type Agrobacterium agrobacterium CP4 mainly include: the application of plant-favored codons; the transformation of unstable sequences; the increase of GC content, etc. The G+C content of the wild-type CP4 strain EPSPS gene is very low, and the A+T content is very high. On the one hand, if these sequences are introduced into the plant genome, they may be mistaken for plant gene control sequences (A+ of known sequ...

Embodiment 2

[0064] Embodiment 2, the acquisition and performance analysis of transgenic cotton

[0065] 1. Obtaining genetically modified cotton

[0066] 1. Obtaining genetically modified cotton

[0067] The recombinant bacteria LBA4404 / pBI121-EPSPS, LBA4404 / pBI121-ACP1-EPSPS and LBA4404 / pBI121-TS-EPSPS transformed into cotton (No. Zhongmian No. 35, hereinafter also referred to as wild-type cotton) in Example 1, specifically transformed Method and screening reference of transgenic cotton plants (Wu JH, Zhang XL, Nie YC, Luo, XY. High-efficiency.transformation of Gossypium hirsutum embryogenic calli.mediated by Agrobacterium tumefaciens and regeneration of insect-resistant plants.2005, Plant Breed.124 : 142-146), to obtain T0 generation EPSPS cotton, T0 generation transfer TS-EPSPS cotton and T0 generation transfer ACP1-EPSPS cotton.

[0068] The same method was used to transform the empty vector pBI121 into wild-type cotton to obtain the T0 generation transformed cotton (EV).

[0069] ...

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Abstract

The invention discloses artificially synthesized herbicide-resistant protein. The invention provides protein. The protein is (1) or (2) as follows: (1) protein composed of an amino acid sequence as shown in a sequence 2 of a sequence table; and (2) protein which is obtained by substituting and / or deleting and / or adding one or more amino acid residues for the amino acid sequence as shown in the sequence 2 of the sequence table, has the same function and is derived from (1). Experiments prove that the herbicide resistance of a plant into which a fusion gene TS-EPSPS is introduced is higher thanthat of a plant into which an optimized gene of EPSPS is introduced. Therefore, the artificially synthesized herbicide-resistant protein has a wide application prospect in the field of herbicide resistance of plants.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to an artificially synthesized herbicide-resistant protein. Background technique [0002] For a long time, weeds have been very serious in agricultural production, and about 67% of cotton fields suffer from weeds every year. Affected by our country's farming methods, the control of weeds in the field mainly depends on manual work. Cotton generally needs to be weeded 4-5 times from seedling emergence to before closing. Cotton is one of the important economic crops in our country. Reducing production cost has a very important role in promoting farmers' enthusiasm for cotton planting and ensuring cotton production. It is of great significance to the cotton industry to achieve the purpose of improving weed control efficiency and simplifying production by cultivating herbicide-resistant cotton varieties. [0003] Weeds have strong adaptability and competition for survival, and they constantly...

Claims

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Application Information

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IPC IPC(8): C07K19/00C12N9/10C12N15/62C12N15/82A01H5/00A01H6/60
CPCC12N9/1092C12N15/8221C12N15/8275C12Y205/01019C07K2319/02
Inventor 吴家和胡广
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI