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Cell carcinoma antigen hybridoma cell strain and application method of monoclonal antibody thereof

A hybridoma cell line and monoclonal antibody technology, applied in the field of cells, can solve problems such as differences in action, achieve high sensitivity, and reduce production costs

Pending Publication Date: 2021-01-29
杭州华葵金配生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although highly homologous, their effects are quite different

Method used

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Embodiment Construction

[0026] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are part of the present invention Examples, not all examples. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0027] An application method of a cell cancer antigen hybridoma cell line and its monoclonal antibody, the application method of the cell cancer antigen hybridoma cell line and its monoclonal antibody comprises the following steps:

[0028] S1: First prepare monoclonal antibody 4H8; according to the human SCCA1 and SCCA2 gene and protein sequences on GenBank (accession numbers: N...

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Abstract

The invention discloses a cell carcinoma antigen hybridoma cell strain and an application method of a monoclonal antibody of the cell carcinoma antigen hybridoma cell strain. The antibody for detecting SCC is an antigen polypeptide sequence specifically designed according to the sequence characteristics of SCCA1 and SCCA2, and is used as an antigen to immunize BALB / c mice, a hybridoma cell strain4H8 for generating the antibody is obtained by using a cell fusion technology, and the antibody secreted by hybridoma cells is IgG1 positive; the invention provides the hybridoma cell strain secretingthe monoclonal antibody, the preservation number of the hybridoma cell strain is CCTCC NO: C2019315, and the hybridoma cell strain can stably secrete a high-titer monoclonal antibody for resisting squamous cell carcinoma antigen. An immune reagent developed by using the squamous cell carcinoma antigen-antibody provided by the invention can accurately determine squamous cell carcinoma antigens ina serum sample, can simultaneously detect SCCA1 and SCCA2, has high sensitivity and accuracy, has high correlation with detection results of imported reagents sold on the market, and can replace imported antibodies, so that the production cost is reduced.

Description

technical field [0001] The invention belongs to the field of cell technology, in particular to a method for applying a cell cancer antigen hybridoma cell line and a monoclonal antibody thereof. Background technique [0002] Squamous cell carcinoma antigen (SCC, squamous cell carcinoma antigen) is a tumor marker with good specificity and the earliest diagnosis of squamous cell carcinoma. It is the subunit of squamous epithelium-associated antigen TA-4 isolated from cervical squamous epithelium by Kato in 1977. It is a glycoprotein with a molecular weight of 48KD. It includes two genes, SCCA1 and SCCA2. SCCA1 and SCCA2 share 98% homology, encoding neutral SCCA1 and acidic SCCA2 glycoproteins with 92% homology, respectively, but mainly the former. The former mainly exists in the squamous epithelium in the non-disease state, or it is highly expressed in squamous cell carcinoma tissues, and the latter is mainly expressed around the tumor. At the same time, SCCA2 is also present...

Claims

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Application Information

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IPC IPC(8): C12N5/20C07K16/30G01N33/574G01N33/543G01N33/577
CPCC07K16/30G01N33/57407G01N33/543G01N33/577C07K2317/14
Inventor 罗海峰王文峰奚建红万欢陈洲
Owner 杭州华葵金配生物科技有限公司
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