Kit for jointly detecting three rheumatism items and preparation method of kit

Abstract

The invention relates to the technical field of biology, and in particular, relates to a kit for jointly detecting three rheumatism items and a preparation method of the kit. The bottom of a clampingshell is provided with a bottom plate; an NC membrane is arranged in the middle of the bottom plate; a combination pad is arranged at the end part of the left side of the NC membrane; a sample pad ismounted on the left side of the combination pad; a water absorption pad is arranged at the end part of the left side of the NC membrane; an RF detection line, an ASO detection line, a CRP detection line and a quality control line are sequentially arranged on the NC membrane; a sample adding hole and an observation opening are formed in the upper surface of the clamping shell; the preparation process of the kit comprises the steps: carrying out proportioning reaction on a coating solution, a confining liquid, a microsphere resuspension and a combination pad treatment liquid, then carrying out microsphere activation and related latex treatment, and then carrying out related region spraying and other multi-step treatment to finally complete the preparation process. According to the technicalscheme, the kit for joint detection of three rheumatism items and the preparation method thereof can simultaneously detect multiple types of rheumatism items, are high in detection efficiency, simpleto operate and easy for result observation and identification.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a kit for combined detection of three items of rheumatism and a preparation method thereof. Background technique [0002] Rheumatoid factor (RF) is an autoantibody with denatured IgG as the target antigen. Rheumatoid arthritis (RA) patients and about 50% of healthy people have B cell clones that produce RF, such as denatured IgG or Epstein-Barr virus Under the direct action of pathological factors, etc., a large amount of RF can be synthesized. RF has poor binding ability to natural IgG, but it is easy to react with IgG or polymerized IgG in immune complexes. There are 5 types of RF: IgG, IgA, IgM, IgD, and IgE. The detection of RF is of great significance to the diagnosis, typing and observation of curative effect of rheumatoid arthritis (RA). The three inspections, in addition to the detection of RF indicators, also need to detect the ASO and CRP indicators at the same time. Sinc...

AI Technical Summary

Problems solved by technology

RF has poor binding ability to natural IgG, but it is easy to react with IgG or polymerized IgG in immune complexes

There are 5 types of RF: IgG, IgA, IgM, IgD, and IgE. The detection of RF is of great significance to the diagnosis, typing and observation of curative effect of rheumatoid arthritis (RA). The three inspections, in addition to the detection of RF indicators, also need to detect the ASO and CRP indicators at the same time. Since the same patient needs to perform the three indicators detection respectively, the operation is time-consuming and laborious, and the waiting time for the test results is long, and the detection efficiency is low.

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