Culture method of nardostachys jatamansi callus cells

A technology of callus and culture method, which is applied in the field of culture of Nardinus callus cells, can solve the problems of complicated disinfection and sterilization steps and easy damage to Nardinus plants, and achieve the effects of short cycle, low cost and fast reproduction

Active Publication Date: 2021-02-02
JALA GROUP CORPORATION
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the international endangered plant of N. pine, the project only uses the rhizome and buds of N. pine as explants. Not only is it easy to damage the N. pine plant, b

Method used

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  • Culture method of nardostachys jatamansi callus cells
  • Culture method of nardostachys jatamansi callus cells
  • Culture method of nardostachys jatamansi callus cells

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Embodiment Construction

[0021] The experimental methods used in the following examples are conventional methods unless otherwise specified.

[0022] The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

[0023] Experiment preparation:

[0024] Take one of the medium MS, 1 / 2MS, WPM, B5, N6, with different plant hormones α-naphthaleneacetic acid (α-NAA), 6-benzylaminoadenine (6-BA), 2,4-dichloro Phenoxyacetic acid (2,4-D), made fresh medium according to a certain ratio, added 30g / L sucrose, 7g / L agar, pH5.7-5.8, sterilized in a sterilizing pot at 121°C for 20min for later use .

[0025] The culture conditions of all plant explants are temperature 20±1℃, humidity 60%~70%, light 12 hours a day, and light intensity 1500Lx~2000Lx.

[0026] Callus induction rate = number of induced explants / number of inoculated explants × 100%

[0027] Proliferation coefficient = weight of callus after value-added / weight of callus before value-adde...

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Abstract

The invention belongs to the field of tissue culture, and particularly relates to a culture method of nardostachysjatamansi callus cells. The culture method comprises the following steps: A, washing and disinfecting petioles and leaves of wild nardostachys jatamansi to obtain base tissue; B, cutting the base tissue prepared in the step A, and inoculating a callus induction culture medium with thecut base tissue for culturing to obtain callus; and C, inoculating a callus subculture medium with the callus prepared in the step B for subculture to obtain subculture callus cells. According to theculture method, the leaves and petioles of the nardostachys jatamansi plant are used for inducing the callus cells of the nardostachys jatamansi plant, and the callus cells with high secondary metabolite (chlorogenic acid and polyphenol) content are obtained under low-temperature stimulation induction; and the method can be used for obtaining a large number of nardostachys jatamansi embryogenic callus cells containing active substances in a short time, has the characteristics of safety, no toxicity, short period, high propagation speed, simplicity in operation and low cost, and provides convenience for application of the nardostachys jatamansi extract in actual products.

Description

technical field [0001] The invention belongs to the field of tissue culture, and in particular relates to a culture method of nard pine callus cells. Background technique [0002] Plant cell tissue culture (plant cell and tissue culture) refers to a technique of separating one or several individual cells or a part of a plant body under sterile conditions to undergo dedifferentiation, callus formation, and redifferentiation. Using the plant cell tissue culture method, a large number of secondary metabolites can be extracted from the cultured plant cells or callus under controlled and repeated conditions, and can provide enzyme preparations, pharmaceutical products, and additives for humans. The production of plant secondary metabolites using large-scale plant cell culture technology has been widely used. On the one hand, it can overcome the different growth conditions of the geographical environment, climate, soil, etc., as well as the different time of harvesting medicinal m...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/005A01H4/001Y02P60/14
Inventor 李惠玲蒋耀权章漳
Owner JALA GROUP CORPORATION
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