Microfluidic-chip-based magnetic control capture and separation device and method for two types of pathogenic bacteria

Abstract

The invention provides a microfluidic-chip-based magnetic control capture and separation device and method for two types of pathogenic bacteria. The device comprises superparamagnetic beads, a microfluidic chip (comprising a capture chamber and a separation channel), an electromagnet, a one-way electromagnetic valve, an injection device, a control device and the like, wherein the superparamagneticbeads are different in size, and the surfaces of the superparamagnetic beads are coupled with two types pathogenic bacteria antibodies respectively. Two types of magnetic beads and the pathogenic bacteria are driven to be fully mixed and subjected to immunoreaction by a rotating magnetic field generated by external electromagnets at the upper position and the lower position of the chip capture chamber, magnetic bead / pathogenic bacterium compounds with different sizes are generated respectively, and the two types of magnetic bead / pathogenic bacterium compounds are separated in a time-sharing mode through the electromagnet arranged above a separation channel. According to the device and the method, the difficult problem that two types of pathogenic bacteria are difficult to capture and separate simultaneously in a conventional micro-fluidic chip is solved, the capture and separation efficiency and the automation degree are improved, and a foundation is laid for subsequent high-precisionhigh-efficiency pathogenic bacterium micro-fluidic detection.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a magnetically controlled capture and separation device and method for two pathogenic bacteria. Background technique [0002] The detection of pathogenic bacteria contamination is widely used in many fields such as food and medical treatment. The traditional plate counting method usually has the disadvantages of time-consuming and low efficiency. The detection method of pathogenic bacteria based on microfluidic chip has the advantages of small , detection speed, low detection limit, high degree of automation and many other advantages, it has been widely used in various fields of pathogen detection. However, the channel size of the microfluidic chip belongs to the micro-nano scale, and the laminar flow characteristics of the micro-channel will make it difficult to capture and separate bacteria in the chip, which will seriously affect the accuracy and efficiency of sub...

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Problems solved by technology

[0006] In view of the above problems, the present invention provides a microfluidic chip-based magnetic control capture and separation device and method for two kinds of pathogenic bacteria, which effectively solves the technical problem that the existing magnetic control capture method cannot capture and separate two kinds of pathogenic bacteria at the same time

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