Supercharge Your Innovation With Domain-Expert AI Agents!

Method for producing conidia through artificially inducing Mycocentrospora Acerina

A technology of conidia and artificial induction, applied in the direction of using spores, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problem of impurity of conidia suspension, contamination by other microorganisms, complex operation of traditional methods, etc. problems, to achieve the effect of stable sporulation, low cost and simple operation

Pending Publication Date: 2021-02-05
YUNNAN AGRICULTURAL UNIVERSITY +1
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is that the traditional method is complicated to operate, and is easily polluted by other microorganisms during the cultivation process, and the obtained conidia suspension is not pure

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for producing conidia through artificially inducing Mycocentrospora Acerina
  • Method for producing conidia through artificially inducing Mycocentrospora Acerina
  • Method for producing conidia through artificially inducing Mycocentrospora Acerina

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1: The experimenters collected the diseased leaves of Panax notoginseng round spot disease in Qiubei County, Wenshan Prefecture, Yunnan Province in July 2019, and isolated the pathogenic bacteria by tissue separation method. After culturing the purified pathogenic strain SDM5 on PDA medium for 7 days , cut a square piece of mycelium with a scalpel and put in sterile ddH 2The induction was carried out in O, and after 15 days of alternating light and dark culture in a light incubator at 20°C, sporulation began, and then the amount of sporulation increased.

Embodiment 2

[0044] Example 2: In September 2019, the experimenters collected the diseased leaves of Panax notoginseng round spot in the Linxia Panax notoginseng base of Lancang County, Puer City, isolated and purified the pathogenic strain DLC16, and used the same method as in Example 1 to cultivate Conidia began to be produced after 10 days.

[0045] The present invention proposes a new conidia induction method aimed at the pathogenic bacterium of Panax notoginseng round spot disease (acerus spp.). Contamination by other miscellaneous bacteria can obtain comparatively pure conidia suspension; For sporulation amount, the present invention can produce 2 * 10 in each petri dish on average 4 conidia; the method is low in cost, and general laboratories can meet the requirements of sporulation conditions of the present invention.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for producing conidia through artificially inducing Mycocentrospora Acerina. The method comprises the following steps of separating the Mycocentrospora Acerina out ofsick leaves of Panax Notoginseng infected with dollar spot through using a tissue separation method to obtain a purified culture strain; inoculating the purified strain into a PDA culture medium; culturing the strain for 5-7 days in an illumination incubator at 20 DEG C under a full illumination condition; scraping hyphae on the surface of the cultured Mycocentrospora Acerina through using a sterilized scalpel, and cutting the hyphae into circular bacterial blocks; adding sterile ddH2O into a sterile culture dish, and then putting the bacterial blocks into the ddH2O in a manner that the frontsurfaces of the bacterial blocks face upwards; putting the culture dish filled with the bacterial blocks into an illumination incubator for alternate light and dark culture; and obtaining a large number of conidia after 15 days. According to the new method for conidia inducing, operation is simple, conidia production is stable, the whole process is operated in a sterile environment, and thereforepure conidia suspension can be obtained.

Description

technical field [0001] The invention relates to the technical field of plant protection, in particular to a method for artificially inducing the Acer sporosum to produce conidia. Background technique [0002] The causative agent of Panax notoginseng round spot disease is Mycocentrosporaacerina (Hartig) Deighton, which belongs to the fungus of the genus Echinococcus. The disease was first discovered in Wenshan Prefecture, Yunnan Province in 1993. Some very serious diseases. Acer fungus Echinospora likes low temperature and high humidity, and spreads rapidly. In the area where Panax notoginseng grows, the rainy season comes every year. If the control is not timely, the incidence rate can reach more than 80%, causing serious economic losses to seven farmers. [0003] Acer sporosum survives the winter in the soil or plant residues with chlamydospores, and is the main source of initial infection of Panax notoginseng round spot in the next year. It often occurs and prevails in th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N3/00C12R1/645
CPCC12N3/00
Inventor 何霞红杨宽郭力维朱书生黄红苹魏朝霞叶坤浩刘屹湘黄惠川王慧玲朱有勇
Owner YUNNAN AGRICULTURAL UNIVERSITY
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com