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Kit and method for detecting fructosamine

A kit and fructosamine technology, applied in the field of fructosamine detection based on the nitro blue tetrazolium method, can solve the problems of limited reagent use range and large differences, and achieve the effect of improving the convenience of operation

Pending Publication Date: 2021-02-05
SICHUAN MACCURA BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, the commercially available NBT method kits and the 4th edition of the "National Clinical Testing Procedures" both indicate that serum samples are required for the detection of fructosamine using the NBT method, and at the same time, the reference intervals given are also serum samples This is because, due to certain differences between the components in plasma and serum, the NBT method has the defect that the difference is too large when testing plasma samples
For example, Yan Cui'e and others clarified that there is a difference between the measured values ​​of plasma and serum, so the reagents produced by many reagent manufacturers are limited to the determination of serum samples, which limits the scope of use of the reagents

Method used

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  • Kit and method for detecting fructosamine
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  • Kit and method for detecting fructosamine

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Embodiment 1

[0058] According to the table 1 below, the fructosamine detection reagent of the present invention was prepared, wherein reagent 1 and reagent 2 were liquid components independent of each other.

[0059] Table 1

[0060] Reagent 1: Reagent 2: Carbonate buffer 100mmol / L (pH 7.0) Phosphate buffer 100mmol / L (pH 6.0) Nitrotetrazolium blue 0.1mmol / L Phenolic ether phosphate potassium salt 0.5g / L The solvent is purified water The solvent is purified water

Embodiment 2

[0062] According to the table 2 below, the fructosamine detection reagent of the present invention was prepared, wherein reagent 1 and reagent 2 were liquid components independent of each other.

[0063] Table 2

[0064] Reagent 1: Reagent 2: Carbonate buffer 400mmol / L (pH 9.0) Phosphate buffer 250mmol / L (pH 7.5) Nitro blue tetrazolium 5mmol / L Phenolic ether phosphate potassium salt 10g / L The solvent is purified water The solvent is purified water

Embodiment 3

[0066] According to the following table 3, the fructosamine detection reagent of the present invention was prepared, wherein reagent 1 and reagent 2 were liquid components independent of each other.

[0067] table 3

[0068] Reagent 1: Reagent 2: Carbonate buffer 800mmol / L (pH 11.0) Phosphate buffer 500mmol / L (pH 9.0) Nitro blue tetrazolium 10mmol / L Phenol ether phosphate potassium salt 20g / L The solvent is purified water The solvent is purified water

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Abstract

The present invention relates to a kit for detecting fructosamine. The kit comprises a first reagent containing a carbonate buffer, a second reagent containing nitrotetrazole blue and an anionic surfactant; a calibration product. The anionic surfactant is selected from one or more of a phosphate anionic surfactant and a sulfonate anionic surfactant, and optionally, the kit is used for detecting fructosamine in blood plasma. According to the kit of the invention, the consistency of detection results of serum and plasma samples during fructosamine detection is improved. The invention also relates to a method for detecting fructosamine and application of the anionic surfactant in improving the consistency of fructosamine detection results.

Description

technical field [0001] The invention relates to the field of fructosamine detection, in particular to the detection of fructosamine based on the nitro blue tetrazolium method. Background technique [0002] Fructosamine (FMN) is a derivative produced by the non-enzymatic glycation reaction of glucose in the blood with the N-terminal amino groups of albumin and other protein molecules. Due to the short half-life of glycated albumin (ALB) in the blood (19d), Therefore, it can be used to reflect the average level of blood sugar in the body in the last 2 to 3 weeks, and its concentration level is positively correlated with the blood sugar level. Since FMN has the advantages of convenient laboratory testing, low cost, and not easily affected by diet and drugs, it can be combined with other clinical indicators, for example, to assist in the judgment of the occurrence, progression, and prognosis of diabetes. [0003] Determination of fructosamine has the following methods: affinity...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/77G01N21/31
CPCG01N21/77G01N21/31
Inventor 任荣梅龙腾镶吴昌英陈中洪
Owner SICHUAN MACCURA BIOTECH CO LTD
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