Preparation method of monodisperse polymer fluorescent microspheres

A technology of fluorescent microspheres and polymers, applied in chemical instruments and methods, analytical materials, luminescent materials, etc., can solve problems such as good monodispersity, achieve uniform size distribution, improve weak fluorescence intensity, and regular surface effects

Pending Publication Date: 2021-02-09
ANHUI WEIZHEN BIOLOGICAL ENG TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] At present, there are many reports on the preparation of polymeric carboxyl fluorescent microspheres, but there is still a lack of a simple and effective preparation technology to obtain polymer microspheres with uniform and controllable size, good monodispersity, high fluorescence intensity and stability.

Method used

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  • Preparation method of monodisperse polymer fluorescent microspheres
  • Preparation method of monodisperse polymer fluorescent microspheres
  • Preparation method of monodisperse polymer fluorescent microspheres

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Experimental program
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Embodiment Construction

[0038] Reagents and Instruments

[0039] Europium trichloride hydrate (EuCl3 6H2O), with a purity of 99.9%, was purchased from Qingda Fine Chemical Factory in Yutai County; dibenzoylmethane, o-phenanthroline, styrene, acrylic acid, potassium persulfate, dichloroethyl Alkane and sodium dodecyl sulfonate were purchased from Sinopharm Group.

[0040] Scanning Electron Microscope (SEM), Fourier Transform Infrared Spectrometer (FTIR)

[0041] Synthesis of Europium Ternary Complexes

[0042] Weigh 0.73 g of EuCl3 6H2O and dissolve it in 20 mL of ethanol. Take another 20 mL of ethanol, add 1.34 g of dibenzoylmethane and 0.40 g of o-phenanthroline and place it in an oil bath at 50°C while magnetically stirring. Then slowly drop the ethanol solution of EuCl3 6H2O into the ligand solution, adjust the pH to 6-7 with NaOH, continue the reaction for 1 h, age, filter with suction, wash, and dry to obtain a light yellow europium complex, which was analyzed by elemental analysis and The co...

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Abstract

The invention discloses a preparation method of monodisperse polymer fluorescent microspheres. The preparation method comprises the following steps: (1) synthesizing a europium-containing ternary complex by adopting a coordination reaction; (2) preparing carboxyl polystyrene microspheres by adopting a soap-free emulsion polymerization method; and (3) permeating the europium complex into the functional microspheres by a one-step seed swelling method to obtain the fluorescent carboxyl polystyrene microspheres with fluorescent substances wrapped in the microspheres. Fluorescent functional microspheres are obtained through a one-step swelling method, seed microspheres used for swelling come from carboxyl latex microspheres obtained in a soap-free emulsion polymerization mode, operation is easy, and the obtained microspheres are uniform and controllable in size and regular in surface.

Description

technical field [0001] The invention relates to a preparation method of monodisperse polymer fluorescent microspheres. Background technique [0002] In recent years, polymer microspheres have received extensive research and attention due to their regular and stable morphology, good monodispersity, adjustable specific surface area, narrow particle size distribution, and surface functionalization. , immunoassay and other high-tech fields have been unanimously favored. Such as blood cell antigen detection, blood flow analysis, drug screening, etc. The surface of microspheres is functionalized through the copolymerization reaction of styrene and carboxyl functional monomers, so as to achieve the polymer immune latex that can be marked. However, most of the polymer microspheres produced at home and abroad are milky white, and the contrast is not obvious. visible to the naked eye. Therefore, dyeing or fluorescently marking the microspheres to make the microspheres have color or...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/02C09K11/06G01N33/533G01N33/58
CPCC09K11/025C09K11/06G01N33/582G01N33/587G01N33/533C09K2211/182
Inventor 杨承凤周梦圆
Owner ANHUI WEIZHEN BIOLOGICAL ENG TECH CO LTD
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