Multi-fluorescence detection device

Abstract

The invention discloses a multi-fluorescence detection device which is aligned with a sample and comprises a lens, an annular lighting device, a plurality of light sources, an optical fiber, a camera,an optical filter wheel and a motor, wherein the lens is positioned right above sample and is aligned with sample; the annular lighting device is arranged at the periphery of the lens in a sleeving manner and is provided with a plurality of light-emitting points distributed in an annular mode; the plurality of laser light sources respectively emit laser light; the optical fiber is connected witheach light-emitting point of the annular lighting device and each laser light source; the camera is positioned right above the lens, is aligned with the lens and is used for collecting fluorescence signals; the optical filter wheel is horizontally arranged between the camera and the lens and carries a plurality of optical filters corresponding to different emission wavelengths of the fluorescent substance in an annular array manner; and the motor is used for driving the optical filter wheel to rotate so as to enable one optical filter to be positioned right above the lens. The cross between fluorescence is reduced, and the detection sensitivity and accuracy of the fluorescence are effectively improved.

Description

technical field [0001] The invention relates to the field of fluorescence detection, in particular to a multiple fluorescence detection device. Background technique [0002] At present, in most fields of nucleic acid detection, such as forensic identification, forensic criminal DNA identification, clinical gene variation detection, identification of viruses and bacteria, etc., most methods are to label nucleic acids with fluorescent groups. The combination of target nucleic acids is used to judge the results. For the same detection throughput, multiple targets are usually detected at one time, that is, multiple fluorescence. Therefore, fluorescence excitation and detection are important factors that affect the result judgment. The traditional method uses white light for excitation and light filtering. Filter out other stray light, or use a single light source to excite multiple fluorescence. These methods will form crossover between each fluorescence. When there are few targ...

AI Technical Summary

Problems solved by technology

[0002] At present, in most fields of nucleic acid detection, such as forensic identification, forensic criminal DNA identification, clinical gene variation detection, identification of viruses and bacteria, etc., most methods are to label nucleic acids with fluorescent groups. The combination of target nucleic acids is used to judge the results. For the same detection throughput, multiple targets are usually detected at one time, that is, multiple fluorescence. Therefore, fluorescence excitation and detection are important factors that affect the result judgment. The traditional method uses white light for excitation and light filtering. filter to filter out other stray light, or use a single light source to excite multiple fluorescence, these methods will form a cross between each fluorescence, when there are few targets, it is easy to mistakenly judge the signal as the background

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