Fish protein hydrolysate powder and composition comprising said powder for use as medicament
A technology of fish protein hydrolyzate and composition, which is applied in the field of preventing or treating oxidative damage of the gastrointestinal tract and central nervous system, and protecting the gastrointestinal tract and central nervous system from oxidative damage, and can solve the problem of increased toxicity of metabolites and increased oxidation Metabolite susceptibility, chemotoxic necrotizing colitis, etc.
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Embodiment 1
[0060]Example 1-Preparation of fish hydrolysate powder with biological activity according to the present invention
[0061]Salmon protein hydrolysate powder is produced by enzymatically hydrolyzing the head and backbone of sliced salmon (Atlantic salmon). 1000 grams of ground head and spine were added to 1000 ml of water, and the mixture was heated to 50°C. 10 g of endopeptidase-pepsin was added and stirred for 30 minutes. 10 g of exopeptidase-carboxypeptidase was further added thereto and stirred for 15 minutes. 5 g of flavor protease (R) (protease derived from Aspergillus oryzae) was added thereto, and the mixture was stirred for 10 minutes. The entire mixture was then heated to 85°C, kept at that temperature for 15 minutes and filtered. The hydrolysate fraction is concentrated to 30% dry matter in a conventional evaporator, and spray-dried to produce the fish protein hydrolysate powder of the present invention, which is a light yellow free-flowing powder with the biological charac...
Embodiment 2
[0062]Example 2-Preparation of fish hydrolysate powder without biological effect
[0063]Except that the exopeptidase used was an aminopeptidase instead of a carboxypeptidase, salmon protein hydrolysate powder was produced as described in Example 1 above. A powder with no biological efficacy was obtained (data not shown).
[0064]Details: Salmon protein hydrolysate powder is produced by enzymatic hydrolysis of sliced salmon (Atlantic salmon) head and spine. 1000 grams of ground head and spine were added to 1000 ml of water, and the mixture was heated to 50°C. 10 g of endopeptidase-pepsin was added and stirred for 30 minutes. 10 g of exopeptidase-aminopeptidase was further added thereto and stirred for 15 minutes. 5 g of flavor protease (R) was further added thereto, and the mixture was stirred for 10 minutes. The entire mixture was then heated to 85°C, kept at that temperature for 15 minutes and filtered. The water layer is concentrated to 30% dry matter in a conventional evaporator and...
Embodiment 3
[0065]Example 3-Preparation of fish hydrolysate powder without biological effect
[0066]Except for adding exopeptidase (carboxypeptidase) before the endopeptidase, salmon protein hydrolysate powder was produced as described in Example 1 above. A powder with no biological efficacy was obtained (data not shown).
[0067]Details: Salmon protein hydrolysate powder is produced by enzymatic hydrolysis of sliced salmon (Atlantic salmon) head and spine. 1000 grams of ground head and spine were added to 1000 ml of water, and the mixture was heated to 50°C. Add 10 grams of exopeptidase-carboxypeptidase and stir for 30 minutes. 10 g of endopeptidase-pepsin was further added and stirred for 15 minutes. 5 g of flavor protease (R) was further added thereto, and the mixture was stirred for 10 minutes. The entire mixture was then heated to 85°C, kept at that temperature for 15 minutes and filtered. The water layer is concentrated to 30% dry matter in a conventional evaporator and spray dried to produc...
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