An animal model of laryngeal precancerous lesions and its construction method and application
A technology of animal model and construction method, which is applied in animal husbandry and other fields, can solve the problems of few animal models of precancerous lesions of laryngeal cancer and no successful animal models of precancerous lesions of laryngeal cancer, and achieve good clinical symptom simulation and increase solubility and stability, reducing the effect of interference factors
Active Publication Date: 2022-07-22
THE SECOND HOSPITAL OF TIANJIN MEDICAL UNIV
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Problems solved by technology
However, there are few studies on animal models of precancerous lesions of laryngeal cancer at present, and there is no successful animal model of precancerous lesions of laryngeal cancer
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Embodiment 1
[0036] The present embodiment constructs a laryngeal precancerous lesion animal model, and the method is as follows:
[0037] First, measure the distance from the incisors of the Wistar rat to the bilateral vocal cords and the root of the epiglottis, and determine the depth of the oral smear, which is about 12mm on average. The operation process is as follows: figure 1 A and B are shown.
[0038]Then, 36 10-week-old male Wistar rats were equally divided into 2 groups according to body weight, namely the control group and the model group, with 18 rats in each group. The laryngeal mucosa of Wistar rats in the model group was smeared with a 1% 4NQO dichloromethane mixed solution, once every two days, for 1.5 minutes per rat, for a total of 20 weeks. Wistar rats in the control group were smeared with normal saline on the laryngeal mucosa, once every two days, for 1.5 minutes per rat, for a total of 20 weeks. The smear is as follows: gun-shaped tweezers hold mung bean-sized cotto...
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The invention relates to an animal model of laryngeal precancerous lesions and a construction method and application thereof. The construction method comprises: applying a dichloromethane solution dissolved with 4-nitroquinoline-1-oxide on the mucous membrane of the throat of the animal, The animal model of laryngeal precancerous lesions is obtained. The use of 4NQO combined with dichloromethane has a synergistic carcinogenic effect, which can significantly shorten the time required for successful single-drug modeling; the model is similar to human patients, the laryngeal mucosa is whitish, thorn-like protrusions appear on the surface, or the laryngeal mucosa appears on the surface Plaques and superficial ulcers are highly similar to the patient's performance and have good clinical symptom simulation; secondly, 4NQO can be directly dissolved in dichloromethane to increase its solubility and stability; and the prepared mixture has a certain It can better contact and adhere to the laryngeal mucosa when it is applied to the laryngeal mucosa, and significantly prolong the stimulation time of carcinogens on the laryngeal mucosa; this construction method does not induce gastrointestinal tumors and reduces interference factors.
Description
technical field [0001] The invention belongs to the field of biotechnology, in particular to an animal model of laryngeal precancerous lesions and a construction method and application thereof, in particular to an animal model that simulates human laryngeal precancerous lesions well, and its construction method and application. Background technique [0002] The occurrence, development, invasion and metastasis of cancer is a very complex process, and the same is true for laryngeal cancer, involving the participation of multiple genes and factors, including the activation of oncogenes, the loss of control of apoptotic genes, and the loss of tumor suppressor genes. The result of the interaction of many factors such as inactivation. Early detection and early diagnosis of laryngeal cancer is particularly critical in the treatment of laryngeal tumors. We must understand the pathogenesis of laryngeal cancer and intervene in the state of precancerous lesions as soon as possible, whi...
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IPC IPC(8): A01K67/02
CPCA01K67/02A01K2207/20A01K2227/105A01K2267/0331
Inventor 牛俊涛
Owner THE SECOND HOSPITAL OF TIANJIN MEDICAL UNIV