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Nano preparation for targeted cardiac repair and preparation method thereof

A nano-preparation and targeting technology, applied in the field of biomedical engineering materials, can solve the problems of normal cell toxicity, non-regeneration of cardiomyocytes, damage to heart function, etc.

Active Publication Date: 2021-07-06
SHENZHEN PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Traditional drug therapy and surgical treatment have seriously reduced the mortality rate of myocardial infarction, but the infarcted cardiomyocytes cannot regenerate, thereby impairing the function of the heart
Furthermore, stem cells transplanted in ischemic heart tissue are affected by apoptosis and necrosis due to the oxidative and inflammatory microenvironment of the infarct zone, severely limiting the therapeutic efficiency of stem cell transplantation
Traditional nanomaterials have certain toxic and side effects on normal cells because of their lack of specificity when carrying drugs

Method used

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  • Nano preparation for targeted cardiac repair and preparation method thereof
  • Nano preparation for targeted cardiac repair and preparation method thereof
  • Nano preparation for targeted cardiac repair and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1: Coenzyme Q 10 - Preparation of NEs-TPGS-Arg

[0036] First, add 2g of coenzyme Q 10 Heat and melt in a constant temperature water bath at 60°C to form liquid coenzyme Q 10 Oil phase; subsequently, 2g of soybean phosphatidylcholine, 1g of arginine-modified polyethylene glycol 1000 vitamin E succinate (TPGS-Arg), 70g of glycerin and 25ml of deionized water were placed in a 50°C beaker, heated and stirred at constant temperature Until the soybean phosphatidylcholine is completely dissolved to form an aqueous phase; finally, the liquid coenzyme Q 10 Add the oil phase to the water phase, heat and stir at 50°C for 20 minutes, ultrasonically disperse for 2 hours to form a transparent and clear microemulsion, and obtain coenzyme Q after cooling 10 -NEs-TPGS-Arg, kept airtight.

Embodiment 2

[0037] Embodiment 2: Coenzyme Q 10 Preparation and Characterization of -NEs-TPGS-Arg / Pterostilbene Nanoparticles

[0038] Take 1g of coenzyme Q prepared above 10 -NEs-TPGS-Arg, add 3 mg of pterostilbene, ultrasonically disperse for 5 min, and stir at room temperature for 4 h to obtain a clear and transparent colloidal solution. Characterization of Coenzyme Q by Fourier Infrared Spectroscopy 10 -The characteristic peak changes of NEs-TPGS-Arg / pterostilbene nanoparticles before and after drug loading. FTIR Fourier transform infrared spectroscopy is a spectrum that shows molecular vibrations. It can identify the functional groups in the substance to be tested. The infrared test of the products and raw materials obtained in each step can prove whether the target product has been successfully synthesized. Take an appropriate amount of the substance to be tested (i.e. the coenzyme Q prepared in Example 1) 10 -NEs-TPGS-Arg, the Q10-NES-TPGS / pterostilbene nanoparticle that embodim...

Embodiment 3

[0039] Embodiment 3: coenzyme Q 10 Preparation and Characterization of -NEs-TPGS-Arg / Pterostilbene Nanoparticles

[0040] Take 2g of coenzyme Q prepared above 10 -NEs-TPGS-Arg, add 6mg of pterostilbene, ultrasonically disperse for 5min, and stir at room temperature for 4h to obtain a clear and transparent colloidal solution. Coenzyme Q at different scales detected by transmission electron microscopy 10 - Transmission electron microscope image of NEs-TPGS-Arg / pterostilbene nanoparticles. from figure 2 It can be seen in the figure that the nanoparticles are spherical, the particle size is relatively uniform, the particle size is between 60-80nm, and there is no adhesion to each other.

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Abstract

The invention relates the field of biomedical engineering materials, in particular to a nano preparation for targeted cardiac repair and a preparation method thereof. The nano preparation is prepared from the following raw materials in parts by weight: 0.1-0.5 part of pterostilbene, 0.5-2 parts of arginine modified to copherylpolyethylene glycol 1000 succinate (TPGS-Arg), 1-3 parts of coenzyme Q10, 1-3 parts of soybean lecithin, 60-80 parts of glycerol, and 20-30 parts of deionized water. The nano preparation is a novel nano carrier material (coenzyme Q10-NEs-TPGS-Arg) developed by taking the pterostilbene as a drug model, and can repair myocardium in a targeted way. Thus, the purpose of increasing drug concentration at a lesion location is achieved.

Description

technical field [0001] The present application relates to the field of biomedical engineering materials, in particular to a nano-preparation for myocardial targeted repair and a preparation method thereof. Background technique [0002] Cardiovascular disease, as one of the diseases with the highest number of patients and the highest death rate in the world, has become the number one killer threatening human health. According to summary statistics, there are 290 million patients with cardiovascular diseases in China, equivalent to 2 out of 10 adults suffering from cardiovascular diseases, including 266 million patients with hypertension, 7 million patients with stroke, 2.5 million patients with myocardial infarction, 4.5 million heart failure patients, 5 million cor pulmonale patients, 2.5 million rheumatic heart disease patients and 2 million congenital heart disease patients. The number of people who die from cardiovascular disease is as high as 3.5 million every year, equ...

Claims

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Application Information

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IPC IPC(8): A61K9/51A61K31/09A61K31/355A61K47/60A61P9/00A61P39/06A61K31/122
CPCA61K31/09A61K31/355A61K31/122A61K47/60A61P9/00A61P39/06A61K9/5146A61K2300/00
Inventor 董少红刘峰贺俊波叶钜亨刘华东吴美善梁新剑
Owner SHENZHEN PEOPLES HOSPITAL
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