Ratiometric fluorescent probe for visually and quantitatively detecting lactic acid and application of ratiometric fluorescent probe

A ratiometric fluorescent probe and quantitative detection technology, which is applied in the fields of fluorescence ratiometric technology, biological analysis and detection, and nanomaterials, can solve the problems that lactic acid detection has not achieved great success, so as to improve the determination selectivity and sensitivity, and the detection operation process The effect of simplicity, good water solubility and biocompatibility

Active Publication Date: 2021-02-26
XUZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the implementation of simple optical strategies for lactate detection has not been very successful

Method used

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  • Ratiometric fluorescent probe for visually and quantitatively detecting lactic acid and application of ratiometric fluorescent probe
  • Ratiometric fluorescent probe for visually and quantitatively detecting lactic acid and application of ratiometric fluorescent probe
  • Ratiometric fluorescent probe for visually and quantitatively detecting lactic acid and application of ratiometric fluorescent probe

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Preparation of bCDs / AgNPs nanocomposites, the steps are as follows:

[0031] Preparation of bCDs / AgNPs nanocomposite: This nanocomposite was prepared using a one-pot method. First dissolve 1mg bCDs with 5ml deionized water to a concentration of 0.2mg mL -1 . This solution was transferred to a 25 mL round bottom flask, then 10 mL (1.2 mM) of silver nitrate solution and 2 mL (30 mM) of sodium citrate solution were added. Subsequently, 380 μL (10 mM) sodium borohydride solution was added dropwise, and stirred at room temperature in the dark for 10 min to obtain a brownish-yellow solution, which was placed in a refrigerator at 4 °C for 3 days in the dark to age to obtain the bCDs / AgNPs nanocomposite.

[0032] Preparation of AgNPs (Liu, S.G.; Mo, S.; Han, L., et al., Oxidation etching induced dual-signal response of carbon dots / silver nanoparticles system forratiometric optical sensing of H 2 o 2 and H 2 o 2 -related bioanalysis. Anal ChimActa 2019,1055:81-8...

Embodiment 2

[0033] Example 2 Preparation of bCDs / AgNPs-rQDs ratio fluorescent probe, the steps are as follows:

[0034] The bCDs / AgNPs solution synthesized in Example 1 was concentrated to 1 mL. Take 20 μL of bCDs / AgNPs concentrate in a 2 mL centrifuge tube, and then add 200 μL of rQDs solution. Subsequently, the bCDs / AgNPs-rQDs ratiometric fluorescent probe was obtained by vortexing at room temperature. Then, take 50 μL of different concentrations of H 2 o 2 Incubated with the probe to measure the fluorescence intensity ratio (I 445 / I 645 ). see results Figure 4 , 5 shown.

Embodiment 3

[0035] Example 3 Prepare a ratiometric fluorescent probe for the visual detection of lactic acid, the steps are as follows:

[0036] The steps are as follows: take 50 μL of lactic acid of different concentrations (0, 1, 2, 5, 10, 20, 50, 100 μM), add 2 U of lactate oxidase each, and incubate the reaction system at 37° C. for 90 minutes. Then add it to the ratiometric fluorescent probe reaction system prepared in Example 2, and shake and mix well. Each concentration was operated in triplicate in parallel, and the fluorescence intensity ratio (I 445 / I 645 ). see results Figure 6 shown.

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Abstract

The invention relates to a ratiometric fluorescent probe for visually and quantitatively detecting lactic acid and application of the ratiometric fluorescent probe. The preparation method of the ratiometric fluorescent probe comprises the following steps that: a bCDs/AgNPs nano-composite of silver nanoparticle-coated doped blue fluorescent carbon dots is prepared, and blue fluorescence of bCDs isquenched due to aggregation and the doping of AgNPs; and combining the bCDs/AgNPs nano composite with quantum dots for red fluorescenceemission to form the bCDs/AgNPs-rQDs ratiometric fluorescent probe. The lactic acid generates H2O2 under the action of lactate oxidase, the H2O2 etches the AgNPs to form silver ions; the structure of the compound is damaged to recover blue fluorescence of the bCDs,the formed silver ions quench the red fluorescence of the rQDs, and therefore, the ratio (I445/I645) of fluorescence intensity of the probe changes along with the change of the concentration of the lactic acid, and the change of the ratio of the fluorescence intensity of the probe can cause obvious change of fluorescence color, and therefore, visual detection is realized. The ratiometric fluorescent probe is used for quantitatively determining biomolecules in serum and is high in sensitivity and simple in detection.

Description

technical field [0001] The invention belongs to the fields of nanomaterials, fluorescence ratio technology and biological analysis and detection, and specifically relates to a method for preparing a ratio fluorescent probe for visually detecting lactic acid, and more specifically, a ratio fluorescence probe based on fluorescence inner filter effect and charge transfer effect Probe; the ratio of probe fluorescence intensity (I 445 / I 645 ) changes with the concentration of lactic acid, and the change of the ratio of the fluorescence intensity of the probe will cause the fluorescence color to change obviously, so as to realize the visual detection. Background technique [0002] As a simple and sensitive detection method, fluorescence detection method has been widely used in clinical testing. Ratiometric fluorescent probes express the content of the analyte through the ratio of two fluorescent signals, which overcomes the defect that single fluorescent probes are susceptible ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/02C09K11/65C09K11/88G01N21/64
CPCC09K11/883C09K11/65C09K11/025G01N21/6428G01N2021/6432
Inventor 马云苏王园刘永杰杨冬芝马雨尹加成陈茜茜
Owner XUZHOU MEDICAL UNIV
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