Super-resolution photoacoustic imaging system and method based on saturated absorption effect

Abstract

The invention discloses a super-resolution photoacoustic imaging system and method based on a saturated absorption effect. The system comprises a laser emission module, a laser beam splitting / combining module and a signal acquisition / scanning imaging module; the laser beam splitting / combining module comprises a first beam splitting sheet, a first reflector, a chopper, a second reflector, a secondbeam splitting sheet, an optical delay unit and a vortex slide, wherein the first beam splitting sheet, the first reflector, the chopper, the second reflector and the second beam splitting sheet are connected in sequence to form a first optical branch. The first beam splitting sheet, the optical delay unit, the vortex slide and the second beam splitting sheet are connected in sequence to form a second optical branch; the first beam splitting sheet is connected with the laser emission module, and the second beam splitting sheet is connected with the signal acquisition / scanning imaging module. According to the invention, super-resolution photoacoustic imaging of a target object can be realized, super-resolution microscopy of non-fluorophores can be realized, an imaging sample cannot be damaged, and the authenticity of structural imaging and functional imaging is ensured.

Description

technical field [0001] The invention relates to a super-resolution photoacoustic imaging system and method based on a saturated absorption effect, and belongs to the technical field of signal modulation and demodulation and photoacoustic microscopic imaging. Background technique [0002] Microscopy has played a key role in the life sciences since it entered the stage of history. Optical microscopy, especially fluorescence microscopy, can study the three-dimensional structure of cells and biological tissues, and display specific biomolecules with high specificity through fluorescent labeling. It is these advantages that make microscopy widely used in biological fields of science and medicine. However, the existence of the optical diffraction limit causes the microscope to blur the imaging of objects with spatial features smaller than half the detection wavelength. In the past two decades, super-resolution microscopy has flourished, and various super-resolution microscopy te...

AI Technical Summary

Problems solved by technology

However, like widefield optical microscopy, the lateral resolution of optically resolved photoacoustic microscopy (OR-PAM) is also limited by optical diffraction.

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